anti-Human Collagen IV alpha4 Antibody | anti-COL4A4 antibody

Collagen IV alpha4 Antibody

Cat. Num. AAA853482

• Gene Names: COL4A4; CA44
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Collagen IV alpha4; Collagen IV alpha4 Antibody; Collagen alpha-4(IV) chain; CO4A4; anti-COL4A4 antibody

• Reactivity: Human
• Specificity: Collagen IV alpha4 antibody detects endogenous levels of total Collagen IV alpha4 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1690

• Applicable Applications for anti-COL4A4 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; IF: 1:100-1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human Collagen IV alpha4.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using Collagen IV alpha4 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of COS-7 cells, using Collagen IV ?4 antibody. )

Product Categories/Family for anti-COL4A4 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Leinonen A., J. Biol. Chem. 269:26172-26177(1994). Boye E., Am. J. Hum. Genet. 63:1329-1340(1998). Hillier L.W., Nature 434:724-731(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 116256356
• NCBI GeneID: 1286
• NCBI Accession #: NP_000083.3
• NCBI GenBank Nucleotide #: NM_000092.4
• UniProt Accession #: P53420; Q53RW9; Q53S42; Q53WR1; A8MTZ1
• Molecular Weight: 164,038 Da
• NCBI Official Full Name: collagen alpha-4(IV) chain
• NCBI Official Synonym Full Names: collagen type IV alpha 4 chain
• NCBI Official Symbol: COL4A4
• NCBI Official Synonym Symbols: CA44
• NCBI Protein Information: collagen alpha-4(IV) chain
• UniProt Protein Name: Collagen alpha-4(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A4

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.