Rabbit anti-Human NDFIP1 Polyclonal Antibody | anti-NDFIP1 antibody

NDFIP1, ID (NDFIP1, N4WBP5, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activatin

Cat. Num. AAA649385

• Gene Names: NDFIP1; N4WBP5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NDFIP1; Polyclonal Antibody; ID (NDFIP1; N4WBP5; NEDD4 family-interacting protein 1; Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activatin; Anti -NDFIP1; anti-NDFIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NDFIP1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: NDFIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-106 amino acids from the Central region of human NDFIP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NDFIP1 Antibody (Center) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the NDFIP1 antibody detected the NDFIP1 protein (arrow).)

Related Product Information for anti-NDFIP1 antibody

The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins.

Product Categories/Family for anti-NDFIP1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 13386480
• NCBI GeneID: 80762
• NCBI Accession #: NP_085048.1
• NCBI GenBank Nucleotide #: NM_030571.3
• UniProt Accession #: Q9BT67; Q658T8; Q8N2E3; Q8N2F9; B2RDB8; D3DQF0
• Molecular Weight: 24,899 Da
• NCBI Official Full Name: NEDD4 family-interacting protein 1
• NCBI Official Synonym Full Names: Nedd4 family interacting protein 1
• NCBI Official Symbol: NDFIP1
• NCBI Official Synonym Symbols: N4WBP5
• NCBI Protein Information: NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; breast cancer-associated protein SGA-1M; putative NFKB and MAPK-activating protein; putative NF-kappa-B-activating protein 164
• UniProt Protein Name: NEDD4 family-interacting protein 1
• Protein Family: NEDD4 family-interacting protein
• UniProt Gene Name: NDFIP1
• UniProt Synonym Gene Names: N4WBP5
• UniProt Entry Name: NFIP1_HUMAN

NCBI Description

The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation

By similarity. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. Ref.11 Ref.12 Ref.13

Subunit structure: Forms heterodimers with NDFIP2. Interacts with several E3 ubiquitin-protein ligases, including NEDD4, NEDD4L and WWP2. Interacts with ITCH, U2SURP and WWP2

By similarity. The interaction with NEDD4, NEDD4L and ITCH leads to relocalization of these proteins to exosomes and eventually to exosomal secretion. Interacts with SLC11A2/DMT1. Interacts with PTEN. May interact with phosphorylated EGFR. Ref.9 Ref.12 Ref.13

Subcellular location: Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane

By similarity. Secreted. Note: Detected in exosomes and secreted via the exosomal pathway. Ref.9 Ref.10 Ref.13

Tissue specificity: Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung. Ref.8

Induction: Increased protein expression in neuronal cells in response to Co2+ or Fe2+ ions. Ref.12

Domain: The PY (WW-binding) motifs are required for E3 ubiquitin-protein ligase binding and activation and for ubiquitination.

Post-translational modification: Ubiquitinated by NEDD4 and ITCH; mono-, di- and polyubiquitinated forms are detected. Ubiquitination regulates its degradation. Ref.11Undergoes transient tyrosine phosphorylation following EGF stimulation, most probably by catalyzed by SRC. Phosphorylation SRC is enhanced in the presence of NDFIP2 which may act as a scaffold to recruit SRC to NDFIP1.