Mouse NFIB NF1B2 Monoclonal Antibody | anti-NFIB antibody

NFIB NF1B2 Monoclonal Antibody

Cat. Num. AAA3014502

• Gene Names: NFIB; CTF; MACID; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: ProG affinity purified.
• Synonyms: NFIB NF1B2; Monoclonal Antibody; NFIB NF1B2 Monoclonal Antibody; NF1-B; Nuclear factor 1/B; CTF; NF-I/B; NFI-B; NFIB; CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; HMGIC/NFIB; NF1B; NF1B2; NFI-RED; Nfib; NFIB2; NFIB3; Nuclear factor 1 B-type; Nuclear Factor 1B; Nuclear Factor I B; Nuclear factor I/B; TGGCA Binding Protein; TGGCA-binding protein; TRANSCRIPTION FACTOR NFIB; anti-NFIB antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: NFIB NF1B2 monoclonal antibody detects endogenous levels of NFIB NF1B2 protein.
• Purity/Purification: ProG affinity purified.
• Form/Format: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 420

• Applicable Applications for anti-NFIB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant full length protein corresponding to human NFIB/NF1B2.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining NFIB/NF1B2 (green) in LO-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-NFIB antibody

NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

NCBI and Uniprot Product Information

• NCBI GI #: 45644934
• NCBI GeneID: 4781
• UniProt Accession #: O00712; P97863
• Molecular Weight: 47kDa
• NCBI Official Full Name: Nuclear factor 1 B-type
• NCBI Official Synonym Full Names: nuclear factor I B
• NCBI Official Symbol: NFIB
• NCBI Official Synonym Symbols: CTF; MACID; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
• NCBI Protein Information: nuclear factor 1 B-type
• UniProt Protein Name: Nuclear factor 1 B-type
• Protein Family: Nuclear factor
• UniProt Gene Name: NFIB
• UniProt Synonym Gene Names: NF1-B; Nuclear factor 1/B; CTF; NF-I/B; NFI-B
• UniProt Entry Name: NFIB_HUMAN

Uniprot Description

NFI-B: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: DNA binding; double-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; pontine nucleus development; glial cell differentiation; anterior commissure morphogenesis; chondrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; DNA replication; negative regulation of DNA binding; hindbrain development