Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Kidney Tissue; Primary Ab: 40ug/ml Mouse Anti-Human NRGN AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse anti-Human Neurogranin (NRGN) Monoclonal Antibody | anti-NRGN antibody
Monoclonal Antibody to Neurogranin (NRGN)
Gene Names
NRGN; RC3; hng
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Neurogranin (NRGN); Monoclonal Antibody; Monoclonal Antibody to Neurogranin (NRGN); anti-NRGN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
The antibody is a mouse monoclonal antibody raised against NRGN. It has been selected for its ability to recognize NRGN in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Length
78
Applicable Applications for anti-NRGN antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Conjugation
None
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Kidney Tissue; Primary Ab: 40ug/ml Mouse Anti-Human NRGN AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Kidney Tissue; Primary Ab: 40ug/ml Mouse Anti-Human NRGN AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-NRGN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neurogranin
NCBI Official Synonym Full Names
neurogranin
NCBI Official Symbol
NRGN
NCBI Official Synonym Symbols
RC3; hng
NCBI Protein Information
neurogranin
UniProt Protein Name
Neurogranin
Protein Family
UniProt Gene Name
NRGN
UniProt Synonym Gene Names
Ng
NCBI Description
Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. [provided by RefSeq, Jul 2008]
Uniprot Description
Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium ().
Research Articles on NRGN
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Product Notes
The NRGN nrgn (Catalog #AAA2111129) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Neurogranin (NRGN) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neurogranin (NRGN) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the NRGN nrgn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurogranin (NRGN), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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