Chemiluminescence
((0.1ug/ml)MBS623565 staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Neurogranin, NRGN Polyclonal Antibody | anti-NRGN antibody
Neurogranin, NRGN (Protein Kinase C Substrate, RC3)
Gene Names
NRGN; RC3; hng
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by peptide affinity chromatography.
Synonyms
Neurogranin; NRGN; Polyclonal Antibody; NRGN (Protein Kinase C Substrate; RC3); Anti -Neurogranin; anti-NRGN antibody
Host
Goat
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Neurogranin. Species crossreactivity
Purity/Purification
Affinity Purified
Purified by peptide affinity chromatography.
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-NRGN antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Peptide ELISA Titer: 1:8000
Western Blot: 0.1-0.3ug/ml detects ~15kD band observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates (calculated MW of 7.6kD according to NP_006167.1). There is also an additional 16kD band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300;
Dilution: Peptide ELISA Titer: 1:8000
Western Blot: 0.1-0.3ug/ml detects ~15kD band observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates (calculated MW of 7.6kD according to NP_006167.1). There is also an additional 16kD band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300;
Immunogen
Synthetic peptide corresponding to C-KIKSGERGRKG, from the internal region of human Neurogranin (NP_006167.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
((0.1ug/ml)MBS623565 staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
((0.1ug/ml)MBS623565 staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-NRGN antibody
Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects.
Product Categories/Family for anti-NRGN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
7,618 Da
NCBI Official Full Name
neurogranin
NCBI Official Synonym Full Names
neurogranin (protein kinase C substrate, RC3)
NCBI Official Symbol
NRGN
NCBI Official Synonym Symbols
RC3; hng
NCBI Protein Information
neurogranin; ng; OTTHUMP00000231529; calmodulin-binding protein; protein kinase C substrate
UniProt Protein Name
Neurogranin
Protein Family
UniProt Gene Name
NRGN
UniProt Entry Name
NEUG_HUMAN
NCBI Description
Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. [provided by RefSeq]
Uniprot Description
neurogranin: Acts as a third messenger substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium. Belongs to the neurogranin family.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 11q24
Cellular Component: intracellular
Molecular Function: calmodulin binding
Biological Process: nervous system development; signal transduction
Research Articles on NRGN
Similar Products
Product Notes
The NRGN nrgn (Catalog #AAA623565) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Neurogranin, NRGN (Protein Kinase C Substrate, RC3) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurogranin, NRGN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Peptide ELISA Titer: 1:8000 Western Blot: 0.1-0.3ug/ml detects ~15kD band observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates (calculated MW of 7.6kD according to NP_006167.1). There is also an additional 16kD band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300;. Researchers should empirically determine the suitability of the NRGN nrgn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurogranin, NRGN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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