Mouse NeuN Monoclonal Antibody | anti-NeuN antibody

NeuN

Cat. Num. AAA370226

• Reactivity: Human, Mouse, Rat, Chicken, Ferret, Pig
• Synonyms: NeuN; Monoclonal Antibody; anti-NeuN antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Chicken, Ferret, Pig
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: A60

• Intended Use: This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.
• Immunogen: Purified cell nuclei from mouse brain.
• Summary and Explanation: NeuN (Feminizing Locus on X-3, Fox-3, or Hexaribonucleotide Binding Protein-3) is a neuron-specific protein that is present in most Central Nervous System (CNS) and Peripheral Nervous System (PNS) neuronal cell types. NeuN protein distributions are restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain. However, some neurons fail to be recognized by NeuN at all ages, such as INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells.; ; NeuN is widely used to label neurons since the vast majority of neurons are strongly positive. NeuN immunoreactivity becomes obvious as neurons mature, typically after they have downregulated expression of Doublecortin, a marker seen in the earliest stages of neuronal development.
• Localization: Nuclear
• Control: Brain
• Presentation: Anti-NeuN is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
• Precautions: 1. For professional users only. Results should be interpreted by a qualified medical professional.; 2. This product contains <0.1% sodium azide (NaN?) as a preservative. Ensure proper handling procedures are used with this reagent.; 3. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 4. Dispose of unused solution with copious amount of water.; 5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL”
• Preparation and Storage: Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

IHC

(Inset: IHC of NeuN on a FFPE Brain Tissue)

Presentation

Staining Procedure

(1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides. 2. Air dry for 2 hours at 58° C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA. 5. Any of three heating methods may be used: a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes. c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with IHC wash buffer or DI water. 9. Continue IHC staining protocol.)

Performance Characteristics