Mouse anti-Human NETO1 Monoclonal Antibody | anti-NETO1 antibody

NETO1 (Neuropilin (NRP) and Tolloid (TLL)-like 1, BCTL1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor Class A domains protein 1, BTCL1, Neuropilin and Tolloid-like Protein 1)

Cat. Num. AAA607335

• Gene Names: NETO1; BCTL1; BTCL1; FLJ41325
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: NETO1; Monoclonal Antibody; NETO1 (Neuropilin (NRP) and Tolloid (TLL)-like 1; BCTL1; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor Class A domains protein 1; BTCL1; Neuropilin and Tolloid-like Protein 1); Anti -NETO1 (Neuropilin (NRP) and Tolloid (TLL)-like 1; anti-NETO1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9L847
• Specificity: Recognizes human NETO1. Species Sequence Homology: mouse (97%), canine (98%).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

• Applicable Applications for anti-NETO1 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.; Immunohistochemistry: 25ug/ml detects NETO1 in paraffin-embedded normal human cortex sections.
• Immunogen: Recombinant corresponding to aa23-345 of human NETO1 expressed in CHO cells (Q8TDF5).
• Hybridoma: Mouse myeloma cells with B cells obtained from a mouse immunized with purified, CHO cell-derived, recombinant human NETO 1 (aa23-345).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NETO1 antibody

Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina. The 511aa residue mature human NETO1 contains an extracellular domain with two CUB domains (aa41-155 and 172-287), and one class A LDL-receptor segment (aa292-326). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation. over aa23-345, human NETO1 shares 97% and 98% aa identity with mouse and canine NETO1, respectively.

Product Categories/Family for anti-NETO1 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 319918840
• NCBI GeneID: 81832
• NCBI Accession #: NP_001188394.1
• NCBI GenBank Nucleotide #: NM_001201465.1
• UniProt Accession #: Q8TDF5; Q86W85; Q8ND78; Q8TDF4
• Molecular Weight: 60,191 Da
• NCBI Official Full Name: neuropilin and tolloid-like protein 1 isoform 3
• NCBI Official Synonym Full Names: neuropilin (NRP) and tolloid (TLL)-like 1
• NCBI Official Symbol: NETO1
• NCBI Official Synonym Symbols: BCTL1; BTCL1; FLJ41325
• NCBI Protein Information: neuropilin and tolloid-like protein 1; OTTHUMP00000163731; OTTHUMP00000163732; OTTHUMP00000197091; brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1
• UniProt Protein Name: Neuropilin and tolloid-like protein 1
• Protein Family: Neuropilin and tolloid-like protein
• UniProt Gene Name: NETO1
• UniProt Synonym Gene Names: BTCL1
• UniProt Entry Name: NETO1_HUMAN

NCBI Description

This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. A similar gene in mice encodes a protein that plays a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq]

Uniprot Description

NETO1: a transmembrane protein and component of the N-methyl-D-aspartate receptor (NMDAR) complex. Required to maintain the normal level of NMDAR subunits at synapses. The NMDAR complex is a major excitatory ligand-gated ion channel in the central nervous system and a major mediator of synaptic plasticity. Important in the development and/or maintenance of neuronal circuitry. Neto1-deficient mice have a dramatic decrease in the number of NMDA receptors at synapses, impairing learning and synaptic plasticity. Three isoforms of the human protein are produced by alternative splicing. Isoforms 1 and 2 are retina-specific whereas isoform 3 is found in retina as well as at lower levels in adult and fetal brain.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q22.2

Cellular Component: postsynaptic membrane; kainate selective glutamate receptor complex; postsynaptic density; extracellular region; excitatory synapse; cell junction

Molecular Function: ionotropic glutamate receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; visual learning; memory