Mouse Nei Endonuclease VIII-like 3 Monoclonal Antibody | anti-NEIL3 antibody

Nei Endonuclease VIII-like 3 (NEIL3, FLJ10858, FPG2, HFPG2)

Cat. Num. AAA601907

• Gene Names: NEIL3; FGP2; FPG2; NEI3; hFPG2; hNEI3; FLJ10858
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Nei Endonuclease VIII-like 3; Monoclonal Antibody; Nei Endonuclease VIII-like 3 (NEIL3; FLJ10858; FPG2; HFPG2); Anti -Nei Endonuclease VIII-like 3 (NEIL3; anti-NEIL3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C230
• Specificity: Recognizes human Nei Endonuclease VIII-like 3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-NEIL3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human Nei Endonuclease VIII-like 3
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-NEIL3 antibody

Antibodies; Abs to Enzymes; Endonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 119625121
• NCBI GeneID: 55247
• UniProt Accession #: Q8TAT5; Q2PPJ3; Q8NG51; Q9NV95
• Molecular Weight: 67,769 Da
• NCBI Official Full Name: nei endonuclease VIII-like 3 (E. coli)
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 3 (E. coli)
• NCBI Official Symbol: NEIL3
• NCBI Official Synonym Symbols: FGP2; FPG2; NEI3; hFPG2; hNEI3; FLJ10858
• NCBI Protein Information: endonuclease 8-like 3; OTTHUMP00000218810; nei-like protein 3; DNA glycosylase FPG2; DNA glycosylase hFPG2; endonuclease VIII-like 3; DNA glycosylase/AP lyase Neil3
• UniProt Protein Name: Endonuclease 8-like 3
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL3
• UniProt Entry Name: NEIL3_HUMAN

NCBI Description

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM]

Uniprot Description

NEIL3: Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single- stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected. Belongs to the FPG family.

Protein type: EC 4.2.99.18; DNA-binding

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: nucleus

Molecular Function: DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; DNA N-glycosylase activity; double-stranded DNA binding; damaged DNA binding; bubble DNA binding; single-stranded DNA binding

Biological Process: nucleotide-excision repair; base-excision repair; DNA catabolic process, endonucleolytic