Testing Data
Mouse anti-Human NDC80 Homolog, Kinetochore Complex Component Monoclonal Antibody | anti-NDC80 antibody
NDC80 Homolog, Kinetochore Complex Component (HEC, HEC1, KNTC2, hsNDC80, TID3)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Testing Data
Testing Data
Western Blot (WB)
(Detection of human KNTC2 by Western blot. Samples: Whole cell lysate (25 μg) from HeLa cells. Predicted molecular weight: 73 kD)
Western Blot (WB)
(Detection of human KNTC2 by Western blot. Samples: Whole cell lysate (25 μg) from HeLa cells. Predicted molecular weight: 73 kD)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-KNTC2 mAb. Nuclear was stained with Hoechst (blue fluorescence).)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-KNTC2 mAb. Nuclear was stained with Hoechst (blue fluorescence).)
NCBI and Uniprot Product Information
NCBI Description
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
Uniprot Description
HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 18p11.32
Cellular Component: kinetochore; membrane; cytosol; nucleus; chromosome, pericentric region
Molecular Function: protein binding; structural constituent of cytoskeleton
Biological Process: mitosis; mitotic spindle organization and biogenesis; attachment of spindle microtubules to kinetochore during mitosis; establishment of mitotic spindle orientation; mitotic sister chromatid segregation; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation
Research Articles on NDC80
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Product Notes
The NDC80 ndc80 (Catalog #AAA603257) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NDC80 Homolog, Kinetochore Complex Component (HEC, HEC1, KNTC2, hsNDC80, TID3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDC80 Homolog, Kinetochore Complex Component can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Western Blot and Immunocytochemistry. Researchers should empirically determine the suitability of the NDC80 ndc80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDC80 Homolog, Kinetochore Complex Component, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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