Western Blot (WB)
((2ug/ml) staining of Cerebral Cortex ysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat polo-like kinase 5 Polyclonal Antibody | anti-PLK5 antibody
Goat anti-polo-like kinase 5 (aa309-322) Antibody
Gene Names
PLK5; PLK-5; PLK5P; SgK384ps
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
polo-like kinase 5; Polyclonal Antibody; Goat anti-polo-like kinase 5 (aa309-322) Antibody; PLK5; PLK-5; PLK5P; SgK384ps; inactive serine/threonine-protein kinase PLK5; pseudogene; PLK5 antibody; polo-like kinase 5 antibody; PLK-5 antibody; PLK5P antibody; SgK384ps antibody; inactive serine/threonine-protein kinase PLK5 antibody; pseudogene antibody; polo-like kinase 5 (aa309-322); anti-PLK5 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SLDVPRSHGCAPTT
Sequence Length
336
Applicable Applications for anti-PLK5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 37kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 36.3kDa according to NP_001230008.1). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 37kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 36.3kDa according to NP_001230008.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence SLDVPRSHGCAPTT, from the internal region of the protein sequence according to NP_001230008.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((2ug/ml) staining of Cerebral Cortex ysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Cerebral Cortex ysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,487 Da
NCBI Official Full Name
inactive serine/threonine-protein kinase PLK5
NCBI Official Synonym Full Names
polo like kinase 5
NCBI Official Symbol
PLK5
NCBI Official Synonym Symbols
PLK-5; PLK5P; SgK384ps
NCBI Protein Information
inactive serine/threonine-protein kinase PLK5
UniProt Protein Name
Inactive serine/threonine-protein kinase PLK5
Protein Family
UniProt Gene Name
PLK5
UniProt Synonym Gene Names
PLK5P; PLK-5
UniProt Entry Name
PLK5_HUMAN
Uniprot Description
PLK5: Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleolus; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.21; Cell cycle regulation; Protein kinase, Other; Other group; PLK family; PLK2 subfamily
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cytoplasm; nucleolus
Molecular Function: ATP binding; protein kinase activity
Biological Process: cell division; defense response to tumor cell; G2/M transition DNA damage checkpoint; mitosis; protein amino acid phosphorylation; transformed cell apoptosis
Research Articles on PLK5
Similar Products
Product Notes
The PLK5 plk5 (Catalog #AAA423153) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-polo-like kinase 5 (aa309-322) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's polo-like kinase 5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 37kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 36.3kDa according to NP_001230008.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PLK5 plk5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SLDVPRSHGC APTT. It is sometimes possible for the material contained within the vial of "polo-like kinase 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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