Immunofluorescence (IF)
(Nanog antibody (mAb) (Clone 2E6E1) tested by immunofluorescence. Top: HeLa cells stained with Nanog antibody (mAb). Bottom: Hoechst staining.)
Rat anti-Mouse Nanog Monoclonal Antibody | anti-Nanog antibody
Nanog antibody (mAb)(Clone 2E6E1)
Reactivity
Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Protein G Chromatography
Synonyms
Nanog; Monoclonal Antibody; Nanog antibody (mAb)(Clone 2E6E1); ENK; ecat4; 2410002E02Rik; anti-Nanog antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2E6E1
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Nanog antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
ICC/IF: 2-10ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
ICC/IF: 2-10ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
Immunogen
This antibody was raised against a peptide comprising amino acids 2-25 of mouse Nanog.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Immunofluorescence (IF)
(Nanog antibody (mAb) (Clone 2E6E1) tested by immunofluorescence. Top: HeLa cells stained with Nanog antibody (mAb). Bottom: Hoechst staining.)
Immunofluorescence (IF)
(Nanog antibody (mAb) (Clone 2E6E1) tested by immunofluorescence. Top: HeLa cells stained with Nanog antibody (mAb). Bottom: Hoechst staining.)
Western Blot (WB)
(Nanog antibody (mAb) (Clone 2E6E1) tested by Western blot. Nanog antibody detection by Western blot. The analysis was performed using 30 ug of mouse ES nuclear extract and Nanog antibody at a 2 ug/ml dilution.)
Western Blot (WB)
(Nanog antibody (mAb) (Clone 2E6E1) tested by Western blot. Nanog antibody detection by Western blot. The analysis was performed using 30 ug of mouse ES nuclear extract and Nanog antibody at a 2 ug/ml dilution.)
Related Product Information for anti-Nanog antibody
Short Description: Our Nanog antibody (mAb)(Clone 2E6E1) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Mouse samples.
Background: Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Background: Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Product Categories/Family for anti-Nanog antibody
NCBI and Uniprot Product Information
Molecular Weight
45kDa
Protein Family
Similar Products
Product Notes
The Nanog (Catalog #AAA388984) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Nanog antibody (mAb)(Clone 2E6E1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nanog can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ICC/IF: 2-10ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody. Researchers should empirically determine the suitability of the Nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nanog, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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