Western Blot (WB)
(Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing mouse Naked2, using MBS600773.)
Rabbit anti-Mouse Naked2 Monoclonal Antibody | anti-nkd2b antibody
Naked2 (Nkd2)
Gene Names
nkd2b; nkd2; nkd2l
Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Purity
As reported
Synonyms
Naked2; Monoclonal Antibody; Naked2 (Nkd2); Anti -Naked2 (Nkd2); anti-nkd2b antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
8A19
Specificity
Detects transfected levels of mouse Naked2.
Purity/Purification
As reported
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/mlBSA, 50% glycerol, 0.02% sodium azide.
Applicable Applications for anti-nkd2b antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
IImmunoprecipitation: 1:50
Dilution: Western Blot: 1:1000
IImmunoprecipitation: 1:50
Immunogen
Synthetic peptide (KLH-coupled) corresponding to residues surrounding Gly47 of mouse Naked2.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing mouse Naked2, using MBS600773.)
Western Blot (WB)
(Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing mouse Naked2, using MBS600773.)
Related Product Information for anti-nkd2b antibody
Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif; however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/ -catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/ -catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF- to the basolateral plasma membrane of polarized epithelial cells (6).
Product Categories/Family for anti-nkd2b antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
47,136 Da
NCBI Official Full Name
naked 2b
NCBI Official Synonym Full Names
naked cuticle homolog 2b
NCBI Official Symbol
nkd2b
NCBI Official Synonym Symbols
nkd2; nkd2l
NCBI Protein Information
protein naked cuticle homolog 2-like
UniProt Protein Name
Protein naked cuticle homolog 2-like
UniProt Gene Name
nkd2l
UniProt Synonym Gene Names
nkd2b; Naked-2-like; Naked-2B
UniProt Entry Name
NKD2L_DANRE
Uniprot Description
Function: Cell autonomous antagonist of both the canonical and non-canonical Wnt signaling pathways
By similarity.
Subcellular location: Cell membrane
By similarity. Cytoplasm
By similarity.
Sequence similarities: Belongs to the NKD family.Contains 1 EF-hand domain.
Similar Products
Product Notes
The nkd2b nkd2l (Catalog #AAA600773) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Naked2 (Nkd2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Naked2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 IImmunoprecipitation: 1:50. Researchers should empirically determine the suitability of the nkd2b nkd2l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Naked2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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