Rabbit anti-Human Axin2 Monoclonal Antibody | anti-AXIN2 antibody

Axin2

Cat. Num. AAA600927

• Gene Names: AXIN2; AXIL; MGC10366; MGC126582; DKFZp781B0869
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Supernatant; Supernatant
• Synonyms: Axin2; Monoclonal Antibody; Anti -Axin2; anti-AXIN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7H7
• Specificity: Recognizes endogenous levels of total human Axin2 protein at ~95, 98kD.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0..02% sodium azide, 50% glycerol.

• Applicable Applications for anti-AXIN2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Pro566 of human Axin2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of HCT15 and SW620 cell lysates using MBS600927.)

Related Product Information for anti-AXIN2 antibody

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 complexes with APC, GSK3 beta, Dvl and beta-catenin and promotes the GSK3 beta-mediated phosphorylation and subsequent degradation of beta-catenin. Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling. In addition to its role in the Wnt signaling pathway, Axin1 complexes with MEKK1 and causes the activation of c-Jun amino-terminal kinase (JNK/SAPK). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice. Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop.

Product Categories/Family for anti-AXIN2 antibody

Antibodies; Abs to Wnt Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6653584
• NCBI GeneID: 8313
• UniProt Accession #: Q5H9R8; Q9BRF5; Q9Y2T1
• Molecular Weight: 93,542 Da
• NCBI Official Full Name: AXIN2
• NCBI Official Synonym Full Names: axin 2
• NCBI Official Symbol: AXIN2
• NCBI Official Synonym Symbols: AXIL; MGC10366; MGC126582; DKFZp781B0869
• NCBI Protein Information: axin-2; conductin; axin-like protein; axis inhibition protein 2
• UniProt Protein Name: Putative uncharacterized protein DKFZp781B0869
• Protein Family: Axin
• UniProt Gene Name: DKFZp781B0869
• UniProt Entry Name: Q5H9R8_HUMAN

NCBI Description

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq]

Uniprot Description

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q24.1

Cellular Component: Golgi apparatus; centrosome; postsynaptic density; cytoplasmic membrane-bound vesicle; cell cortex; beta-catenin destruction complex; cytosol; microtubule cytoskeleton; nucleoplasm; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: odontogenesis; negative regulation of cell proliferation; cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; dorsal/ventral axis specification; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity

Disease: Oligodontia-colorectal Cancer Syndrome