Western Blot (WB)
(Western Blot: MBS601828- Western blot analysis of O-GlcNAc expression in 1) HeLa, 2) NTERA-2, 3) PC-12 and 4) COS-7 cell lysates using untreated antibody on the left side and antibody preincubated with 50uM GlcNAc)
Mouse N-Acetylglucosamine, O-linked Monoclonal Antibody
Mouse anti-N-Acetylglucosamine, O-linked
Applications
ELISA, Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
Ascites
Synonyms
N-Acetylglucosamine; O-linked; Monoclonal Antibody; Mouse anti-N-Acetylglucosamine; Anti -N-Acetylglucosamine; anti-N-Acetylglucosamine; O-linked antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
8J157
Specificity
Recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins
Purity/Purification
Ascites
Form/Format
Supplied as a liquid, 0.1% sodium azide.
Applicable Applications for anti-N-Acetylglucosamine, O-linked antibody
ELISA (EL/EIA), Western Blot (WB), Chromatin Immunoprecipitation/Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Dilution:
Chromatin Immunoprecipitation: 1:10-1:500
ELISA: 1:100-1:2000
Immunofluorescence (IC): 1:100-1:200
Immunohistochemistry (paraffin): 1:200
Immunoprecipitation: 1:10-1:500
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Chromatin Immunoprecipitation: 1:10-1:500
ELISA: 1:100-1:2000
Immunofluorescence (IC): 1:100-1:200
Immunohistochemistry (paraffin): 1:200
Immunoprecipitation: 1:10-1:500
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Immunogen
Heat killed, pepsin treated group A streptococci (Streptococcus pyrogenes)
Grade
Ascites
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot: MBS601828- Western blot analysis of O-GlcNAc expression in 1) HeLa, 2) NTERA-2, 3) PC-12 and 4) COS-7 cell lysates using untreated antibody on the left side and antibody preincubated with 50uM GlcNAc)
Western Blot (WB)
(Western Blot: MBS601828- Western blot analysis of O-GlcNAc expression in 1) HeLa, 2) NTERA-2, 3) PC-12 and 4) COS-7 cell lysates using untreated antibody on the left side and antibody preincubated with 50uM GlcNAc)
Immunohistochemistry (IHC)
(Immunohistochemistry-Paraffin: A05258-19E IHC staining of O-GlcNAc in mouse colon using DAB with hematoxylin counterstain.)
Immunohistochemistry (IHC)
(Immunohistochemistry-Paraffin: A05258-19E IHC staining of O-GlcNAc in mouse colon using DAB with hematoxylin counterstain.)
Related Product Information for anti-N-Acetylglucosamine, O-linked antibody
O-GlcNAc (O-linked N-acetylglucosamine) is one of the most abundant posttranslational modifications on nuclear and cytoplasmic proteins. Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-GlcNAc residues attached to serine or threonine residues. This modification occurs via the O-GlcNAc transferase (OGT). There appears to be a competitive modification at these residues. Either the residues are glycosylated or they are phosphorylated. Because of the significance of phosphorylation in cancer research, such competition can be of major interest. O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may also obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling. Recent research has revealed that the O-GlcNAcylation of the EGF repeats of Notch are modified via a novel O-GlcNAc transferase, EOGT1.
Product Categories/Family for anti-N-Acetylglucosamine, O-linked antibody
Similar Products
Product Notes
The N-Acetylglucosamine, O-linked (Catalog #AAA601828) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's N-Acetylglucosamine, O-linked can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Chromatin Immunoprecipitation/Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Dilution: Chromatin Immunoprecipitation: 1:10-1:500 ELISA: 1:100-1:2000 Immunofluorescence (IC): 1:100-1:200 Immunohistochemistry (paraffin): 1:200 Immunoprecipitation: 1:10-1:500 Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the N-Acetylglucosamine, O-linked for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-Acetylglucosamine, O-linked, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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