Mouse anti-Human N-Acetylgalactosamine 4 Sulfate 6-O-Sulfotransferase Monoclonal Antibody | anti-CHST15 antibody

N-Acetylgalactosamine 4 Sulfate 6-O-Sulfotransferase (GalNAc4S-6ST)

Cat. Num. AAA602855

• Gene Names: CHST15; BRAG; KIAA0598; MGC34346; GALNAC4S-6ST; RP11-47G11.1; DKFZp781H1369
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by Protein G affinity chromatography.
• Synonyms: N-Acetylgalactosamine 4 Sulfate 6-O-Sulfotransferase; Monoclonal Antibody; N-Acetylgalactosamine 4 Sulfate 6-O-Sulfotransferase (GalNAc4S-6ST); Anti -N-Acetylgalactosamine 4 Sulfate 6-O-Sulfotransferase (GalNAc4S-6ST); anti-CHST15 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8H167
• Specificity: Recognizes human GalNAc4S-6ST.
• Purity/Purification: Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-CHST15 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Direct ELISA and Western Blot.; Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhGaINAc4S-6ST is ~ 10ng/well.; Western Blot: 1-2ug/ml detection limit for rhGaINAc4S-6ST is ~ 5ng/lane under non-reducing and reducing conditions.
• Immunogen: NS0-derived rhGaINAc4S-6ST. (aa 99 - 561)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CHST15 antibody

N-acetylgalactosamine 4 sulfate 6-O-sulfotransferase (GalNAc4S-6ST) also known as B cell RAG-associated protein (BRAG), is a type II transmembrane Golgi protein that catalyzes the transfer of sulfate onto GalNAc (4 SO4) in chondroitin sulfate A and dermatan sulfate. The 469 aa rhGalNAc4S-6ST predicts a molecular mass of 55kD and migrates as two bands with molecular masses of approximately 68 and 73kD. The amino acid sequence of human GalNAc4S-6ST is 99%, 93%, 88% and 79% identical to that of chimpanzee, rat/mouse, canine, and chicken.

Product Categories/Family for anti-CHST15 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 17026121
• NCBI GeneID: 51363
• UniProt Accession #: Q7LFX5; O60338; O60474; Q86VM4
• Molecular Weight: 64,926 Da
• NCBI Official Full Name: N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase
• NCBI Official Synonym Full Names: carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
• NCBI Official Symbol: CHST15
• NCBI Official Synonym Symbols: BRAG; KIAA0598; MGC34346; GALNAC4S-6ST; RP11-47G11.1; DKFZp781H1369
• NCBI Protein Information: carbohydrate sulfotransferase 15; hBRAG; OTTHUMP00000020689; B-cell RAG-associated gene protein; B cell RAG associated protein (GALNAC4S-6ST); N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase
• UniProt Protein Name: Carbohydrate sulfotransferase 15
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST15
• UniProt Synonym Gene Names: BRAG; GALNAC4S6ST; KIAA0598
• UniProt Entry Name: CHSTF_HUMAN

NCBI Description

Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

CHST15: Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; Transferase; EC 2.8.2.33

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity; 3'-phosphoadenosine 5'-phosphosulfate binding

Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; hexose biosynthetic process