Testing Data
(Human alpha fetoprotein detected in a sandwich ELISA using Mouse anti Human alpha fetoprotein as the capture reagent and Mouse anti Human alpha fetoprotein:HRP as the detection reagent)
Mouse anti-Human ALPHA FETOPROTEIN Monoclonal Antibody
MOUSE ANTI HUMAN ALPHA FETOPROTEIN
Reactivity
Human
Applications
ELISA
Synonyms
ALPHA FETOPROTEIN; Monoclonal Antibody; MOUSE ANTI HUMAN ALPHA FETOPROTEIN; Alpha Fetoprotein antibody|125.7A2; anti-ALPHA FETOPROTEIN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
125.7A2
Specificity
ALPHA FETOPROTEIN
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-ALPHA FETOPROTEIN antibody
ELISA (EIA)
Application Notes
ELISA: Max Dilution: 5ug/ml
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Human alpha fetoprotein detected in a sandwich ELISA using Mouse anti Human alpha fetoprotein as the capture reagent and Mouse anti Human alpha fetoprotein:HRP as the detection reagent)
Testing Data
(Human alpha fetoprotein detected in a sandwich ELISA using Mouse anti Human alpha fetoprotein as the capture reagent and Mouse anti Human alpha fetoprotein:HRP as the detection reagent)
Related Product Information for anti-ALPHA FETOPROTEIN antibody
Mouse anti Human alpha fetoprotein antibody, clone 125.7A2 is a monoclonal antibody recognizing alpha fetoprotein (AFP), a member of the albuminoid protein superfamily, along with albumin and vitamin D binding protein. Members of the albuminoid superfamily comprise molecules with three characteristic globular domains (Mizejewski 1995).
AFP is a ~70kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. AFP acts as a transport protein for various ligands such as bilirubin, fatty acids, steroids and heavy metals. After birth, plasma AFP levels drop rapidly with only trace amounts remaining in healthy adults. Changes in plasma levels of AFP have been documented in a variety of cancers including hepatocellular cancer (Sato et al. 1993).
Mouse anti Human alpha fetoprotein antibody, clone 125.7A2 shows <0.1% reactivity with human serum albumin and IgG. Strong expression of AFP has been noted in the hepatic cell line HepG2.
AFP is a ~70kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. AFP acts as a transport protein for various ligands such as bilirubin, fatty acids, steroids and heavy metals. After birth, plasma AFP levels drop rapidly with only trace amounts remaining in healthy adults. Changes in plasma levels of AFP have been documented in a variety of cancers including hepatocellular cancer (Sato et al. 1993).
Mouse anti Human alpha fetoprotein antibody, clone 125.7A2 shows <0.1% reactivity with human serum albumin and IgG. Strong expression of AFP has been noted in the hepatic cell line HepG2.
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Product Notes
The ALPHA FETOPROTEIN (Catalog #AAA226004) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN ALPHA FETOPROTEIN reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALPHA FETOPROTEIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: Max Dilution: 5ug/ml Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the ALPHA FETOPROTEIN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALPHA FETOPROTEIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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