Testing Data
(Staining of permeabilised human peripheral blood granulocytes with Mouse anti Human MYELOPEROXIDASE:FITC (MBS210766))
Mouse MYELOPEROXIDASE Monoclonal Antibody | anti-MPO antibody
MOUSE ANTI HUMAN MYELOPEROXIDASE:RPE
Does not react with Rat.
N.B Antibody reactivity and working conditions may vary between species.
Does not react with Rat.
N.B Antibody reactivity and working conditions may vary between species.
Flow Cytometry: Maximum Dilution: Neat; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
1% Bovine Serum Albumin
5% Sucrose
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Testing Data
(Staining of permeabilised human peripheral blood granulocytes with Mouse anti Human MYELOPEROXIDASE:FITC (MBS210766))
Testing Data
(Staining of permeabilised human peripheral blood granulocytes with Mouse anti Human MYELOPEROXIDASE:FITC (MBS210766))
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase (MBS212300) following permeabilisation with Leucoperm)
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase (MBS212300) following permeabilisation with Leucoperm)
Testing Data
(Staining of human bone marrow. Formalin fixed, paraffin processed tissue with Mouse anti Human Myeloperoxidase (MBS215187))
Testing Data
(Staining of human bone marrow. Formalin fixed, paraffin processed tissue with Mouse anti Human Myeloperoxidase (MBS215187))
Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots,and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody,clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.
Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003)
NCBI and Uniprot Product Information
NCBI Description
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Uniprot Description
MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.11.2.2; Oxidoreductase
Chromosomal Location of Human Ortholog: 17q23.1
Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule
Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding
Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; response to lipopolysaccharide; response to yeast; defense response; response to oxidative stress; defense response to fungus; aging; negative regulation of apoptosis
Disease: Myeloperoxidase Deficiency; Alzheimer Disease
Research Articles on MPO
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Product Notes
The MPO mpo (Catalog #AAA213662) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN MYELOPEROXIDASE:RPE reacts with Reacts with Dog Does not react with Rat. N.B Antibody reactivity and working conditions may vary between species. and may cross-react with other species as described in the data sheet. AAA Biotech's MYELOPEROXIDASE can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Researchers should empirically determine the suitability of the MPO mpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYELOPEROXIDASE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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