Western Blot (WB)
(Western Blot analysis of Jurkat cell lysate using M9600 at 0.5ug/ml. arrow indicates Myb (~80kD).)
Mouse anti-Human, Mouse Myb Monoclonal Antibody | anti-Myb antibody
Myb (c-myb, C-myb, c-myb_CDS, efg, Myb proto-oncogene protein, v-myb myeloblastosis viral oncogene homolog (avian)) (MaxLight 490)
Gene Names
MYB; efg; Cmyb; c-myb; c-myb_CDS
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Purified by ammonium precipitation gel filtration and ion exchange chromatography.
Synonyms
Myb; Monoclonal Antibody; Myb (c-myb; C-myb; c-myb_CDS; efg; Myb proto-oncogene protein; v-myb myeloblastosis viral oncogene homolog (avian)) (MaxLight 490); anti-Myb antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
0.T.94
Specificity
Recognizes mouse Myb, Mr ~ 80kD, containing C-terminal amino acids from 401-636. Species crossreactivity: human
Purity/Purification
Purified by ammonium precipitation gel filtration and ion exchange chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-Myb antibody
Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Recombinant protein corresponding to mouse Myb purified by SDS.
Conjugate
MaxLight490
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Western Blot (WB)
(Western Blot analysis of Jurkat cell lysate using M9600 at 0.5ug/ml. arrow indicates Myb (~80kD).)
Western Blot (WB)
(Western Blot analysis of Jurkat cell lysate using M9600 at 0.5ug/ml. arrow indicates Myb (~80kD).)
Product Categories/Family for anti-Myb antibody
References
1. Ramsay, R.G., et al., Oncogene Res. 4: 259-269, 1989. 2. Gonda, T.J., et al., EMBO J. 4: 2003-2008, 1985. 3. General references: Allen, R. D., Bender, T. P. and Siu, G. (1999). c-Myb is essential for early T cell development. Genes Dev. 13(9): 1073-8. Medline abstract: 99257267 Aziz, N., et al. (1995). Modulation of c-Myb-induced transcription activation by a phosphorylation site near the negative regulatory domain. Proc. Natl. Acad. Sci. 92(14): 6429-6433. Medline abstract: 95327660 Badiani, P., et al. (1994). Dominant interfering alleles define a role for c-Myb in T-cell development. Genes Dev. 8(7): 770-782. Medline abstract: 95011579 Bin, X. and Lipsick, J. S. (1993). Individual repeats of Drosophila Myb can function in transformation by v-Myb. J. Virol. 67(12): 7332-7339. Medline abstract: 94047348 Coffman, J. A., et al. (1997). SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin embryos. Development 124(23): 4717-4727. Medline abstract: 98088684 DeRocco, S. E., et al. (1997). Ectopic expression of A-myb in transgenic mice causes follicular hyperplasia and enhanced B lymphocyte proliferation. Proc. Natl. Acad. Sci. 94(7): 3240-3244. Medline abstract: 97250521 Ess, K. C., et al. (1995). A central role for a single c-Myb binding site in a thymic locus control region. Mol. Cell. Biol. 15(10): 5707-5715. Medline abstract: 96009598 Facchinetti, V., et al. (1997). Regulatory domains of the A-Myb transcription factor and its interaction with the CBP/p300 adaptor molecules. Biochem J. 324(Pt 3): 729-736. Medline abstract: 97327718 Frampton, J., Ramqvist, T., Graf, T. (1996). v-Myb of E26 leukemia virus up-regulates bcl-2 and suppresses apoptosis in myeloid cells. Genes Dev 10(21): 2720-2731. Medline abstract: 97102673 Fung, S. M., Ramsay, G. and Katzen, A. L. (2002). Mutations in Drosophila myb lead to centrosome amplification and genomic instability. Development 129: 347-359. Medline abstract: 11807028 Golay, J., et al. (1997). Redundant functions of B-Myb and c-Myb in differentiating myeloid cells. Cell Growth Differ. 8(12): 1305-1316. Medline abstract: 98080689 Guo, K., et al. (1999). A myb-related protein required for culmination in Dictyostelium. Development 126(12): 2813-2822 Hedge, S. P., et al. (1998). c-Maf interacts with c-Myb to regulate transcription of an early myeloid gene during differentiation. Mol. Cell. Biol. 18(5): 2729-2737. Medline abstract: 98226545 Hou, D. X., Akimaru, H. and Ishii, S. (1997). Trans-activation by the Drosophila myb gene product requires a Drosophila homologue of CBP. FEBS Lett. 413(1): 60-64. Medline abstract: 97431612 Katzen, A. L., Kornberg, T. B. and Bishop, J. M. (1985). Isolation of the proto-oncogene c-myb from D. melanogaster. Cell 41(2): 449-456. Medline abstract: 85176969 Katzen, A. L. and Bishop, J. M. (1996). myb provides an essential function during Drosophila development. Proc. Natl. Acad. Sci. 93(24): 13955-13960. Medline abstract: 97098502 Katzen, K. L., et al. (1998). Drosophila myb is required for the G2/M transition and maintenance of diploidy. Genes Dev. 12: 831-843. Medline abstract: 98180985 Kowenz-Leutz, E., et al. (1997). The homeobox gene GBX2, a target of the myb oncogene, mediates autocrine growth and monocyte differentiation. Cell 91(2): 185-195. Medline abstract: 98004222 Ku, D. H., et al. (1993). c-myb transactivates cdc2 expression via Myb binding sites in the 5'-flanking region of the human cdc2 gene. J. Biol. Chem. 268(3): 2255-2259. Medline abstract: 93131991 Lam, E. W., Bennett, J. D. and Watson, R. J. (1995). Cell-cycle regulation of human B-myb transcription. Gene 160(2): 277-281. Medline abstract: 95369704 Liu, N., et al. (1996). Cell cycle-regulated repression of B-myb transcription: cooperation of an E2F site with a contiguous corepressor element. Nucleic Acids Res. 24(15): 2905-2910. Medline abstract: 96357121 Lucibello, F. C., et al. (1997). The differential binding of E2F and CDF repressor complexes contributes to the timing of cell cycle-regulated transcription. Nucleic Acids Res. 25(24): 4921-4925. Medline abstract: 98060887 Ly, D. H., Lockhart, D. J., Lerner, R. A. and Schultz, P. G. (2000). Mitotic misregulation and human aging. Science 287: 2486-92. Medline abstract: 10741968 Madan, A., et al. (1995). Bacterial expression, characterization and DNA binding studies on Drosophila melanogaster c-Myb DNA-binding protein. Eur. J. Biochem. 232(1): 150-158. Medline abstract: 96048041 Miglarese, M. R., Halaban, R. and Gibson, N. W. (1997). Regulation of fibroblast growth factor 2 expression in melanoma cells by the c-MYB proto-oncoprotein. Cell Growth Differ. 8(11): 1199-1210. Medline abstract: 98039512 Oelgeschlager, M., et al. (1996). C/EBP, c-Myb, and PU.1 cooperate to regulate the neutrophil elastase promoter. Mol. Cell. Biol. 16(9): 4717-4725. Medline abstract: 96347540 Oh, I. H. and Reddy, E. P. (1997). Murine A-myb gene encodes a transcription factor, which cooperates with Ets-2 and exhibits distinctive biochemical and biological activities from c-myb. J. Biol. Chem. 272(34): 21432-21443. Medline abstract: 97407935 Oh, I. H. and Reddy, E. P. (1998). The C-terminal domain of B-Myb acts as a positive regulator of transcription and modulates its biological functions. Mol. Cell. Biol. 18(1): 499-511. Medline abstract: 98078706 Okada, M., et al. (2002). Myb controls G2/M progression by inducing cyclin B expression in the Drosophila eye imaginal disc. EMBO J. 21: 675-684. Medline abstract: 11847115 Otsuka, H. and Van Haastert, P. J. M. (1998). A novel Myb homolog initiates Dictyostelium development by induction of adenylyl cyclase expression. Genes Dev. 12: 1738-1748. Medline abstract: 98283920 Peters, C. W., et al. (1987). Drosophila and vertebrate myb proteins share two conserved regions, one of which functions as a DNA-binding domain. EMBO J. 6(10): 3085-3090. Medline abstract: 88082681 Postigo, A. A., et al. (1997). c-Myb and Ets proteins synergize to overcome transcriptional repression by ZEB. EMBO J. 16(13): 3924-3934. Medline abstract: 97377031 Raschella, G., et al. (1997). Retinoblastoma-related protein pRb2/p130 and its binding to the B-myb promoter increase during human neuroblastoma differentiation. J. Cell Biochem. 67(3): 297-303. Medline abstract: 98027077 Robinson, C., et al. (1996). Cell-cycle regulation of B-Myb protein expression: specific phosphorylation during the S phase of the cell cycle. Oncogene 12(9): 1855-1864. Medline abstract: 96211391 Sala, A. and Calabretta, B. (1992). Regulation of BALB/c 3T3 fibroblast proliferation by B-myb is accompanied by selective activation of cdc2 and cyclin D1 expression. Proc. Natl. Acad. Sci. 89(21): 10415-10419. Medline abstract: 93066249 Sala, A., et al. (1996). B-myb promotes S phase and is a downstream target of the negative regulator p107 in human cells. J. Biol. Chem. 271(16): 9363-9367. Medline abstract: 96199187 Sala, A., et al. (1997). Activation of human B-MYB by cyclins. Proc. Natl. Acad. Sci. 94(2): 532-536. Medline abstract: 97165056 Schaefer, A., et al. (1996). Ca2+/calmodulin-dependent and -independent down-regulation of c-myb mRNA levels in erythropoietin-responsive murine erythroleukemia cells. The role of calcineurin. J. Biol. Chem. 271(23): 13484-13490. Medline abstract: 96278771 Sitzmann, J., et al. (1995). Expression of mouse c-myb during embryonic development. Oncogene 11(11): 2273-2279. Medline abstract: 96112628 Sitzmann, J., et al. (1996). Expression of B-Myb during mouse embryogenesis. Oncogene 12(9): 1889-1894. Medline abstract: 96211395 Suhasini, M., et al. (1998). cAMP-induced NF-kappaB (p50/relB) binding to a c-myb intronic enhancer correlates with c-myb up-regulation and inhibition of erythroleukemia cell differentiation. Oncogene 15(15): 1859-1870. Medline abstract: 98026826 Tahirov, T. H., et al. (2002). Mechanism of c-Myb-C/EBPß cooperation from separated sites on a promoter. Cell 108: 57-70. Medline abstract: 11792321 Taylor, D., Badiani, P. and Weston, K. (1996). A dominant interfering Myb mutant causes apoptosis in T cells. Genes Dev. 10(21): 2732-2744. Medline abstract: 97102674 Toscani, A., et al. (1997). Arrest of spermatogenesis and defective breast development in mice lacking A-myb. Nature 386(6626): 713-717. Medline abstract: 97263572 Trauth, K., et al. (1994). Mouse A-myb encodes a trans-activator and is expressed in mitotically active cells of the developing central nervous system, adult testis and B lymphocytes. EMBO J. 13(24): 5994-6005. Medline abstract: 95112810 Ying, G. G., et al. (1997). The DNA binding domain of the A-MYB transcription factor is responsible for its B cell-specific activity and binds to a B cell 110-kDa nuclear protein. J. Biol. Chem. 272(40): 24921-24926. Medline abstract: 97460074 Ziebold, U., and Klempnauer, K. H. (1997). Linking Myb to the cell cycle: cyclin-dependent phosphorylation and regulation of A-Myb activity. Oncogene 15(9): 1011-1019. Medline abstract: 97429917
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,341 Da
NCBI Official Full Name
transcriptional activator Myb isoform 3
NCBI Official Synonym Full Names
v-myb myeloblastosis viral oncogene homolog (avian)
NCBI Official Symbol
MYB
NCBI Official Synonym Symbols
efg; Cmyb; c-myb; c-myb_CDS
NCBI Protein Information
transcriptional activator Myb; c-myb8B_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; proto-oncogene c-Myb; c-myb protein (140 AA)
UniProt Protein Name
Transcriptional activator Myb
Protein Family
UniProt Gene Name
MYB
UniProt Entry Name
MYB_HUMAN
NCBI Description
This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]
Uniprot Description
Myb: Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Oncoprotein; DNA-binding
Chromosomal Location of Human Ortholog: 6q22-q23
Cellular Component: nuclear matrix; cytosol
Molecular Function: protein binding; chromatin binding
Biological Process: positive regulation of histone H3-K9 methylation; thymus development; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; homeostasis of number of cells; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; chromatin remodeling; positive regulation of T-helper cell differentiation; regulation of transcription, DNA-dependent; calcium ion transport; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; blood coagulation; embryonic gut development; G1/S transition of mitotic cell cycle
Research Articles on Myb
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Product Notes
The Myb myb (Catalog #AAA6245235) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Myb (c-myb, C-myb, c-myb_CDS, efg, Myb proto-oncogene protein, v-myb myeloblastosis viral oncogene homolog (avian)) (MaxLight 490) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myb can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the Myb myb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myb, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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