Mouse anti-Human, Mouse Myb Monoclonal Antibody | anti-Myb antibody
Myb (c-myb, C-myb, c-myb_CDS, efg, Myb proto-oncogene protein, v-myb myeloblastosis viral oncogene homolog (avian)) (HRP)
Gene Names
MYB; efg; Cmyb; c-myb; c-myb_CDS
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Purified by ammonium precipitation gel filtration and ion exchange chromatography.
Synonyms
Myb; Monoclonal Antibody; Myb (c-myb; C-myb; c-myb_CDS; efg; Myb proto-oncogene protein; v-myb myeloblastosis viral oncogene homolog (avian)) (HRP); anti-Myb antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
0.T.94
Specificity
Recognizes mouse Myb, Mr ~ 80kD, containing C-terminal amino acids from 401-636. Species crossreactivity: human
Purity/Purification
Purified by ammonium precipitation gel filtration and ion exchange chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-Myb antibody
Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Recombinant protein corresponding to mouse Myb purified by SDS.
Conjugate
HRP
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western Blot analysis of Jurkat cell lysate using M9600 at 0.5ug/ml. arrow indicates Myb (~80kD).)
Product Categories/Family for anti-Myb antibody
References
1. Ramsay, R.G., et al., Oncogene Res. 4: 259-269, 1989. 2. Gonda, T.J., et al., EMBO J. 4: 2003-2008, 1985. 3. General references: Allen, R. D., Bender, T. P. and Siu, G. (1999). c-Myb is essential for early T cell development. Genes Dev. 13(9): 1073-8. Medline abstract: 99257267 Aziz, N., et al. (1995). Modulation of c-Myb-induced transcription activation by a phosphorylation site near the negative regulatory domain. Proc. Natl. Acad. Sci. 92(14): 6429-6433. Medline abstract: 95327660 Badiani, P., et al. (1994). Dominant interfering alleles define a role for c-Myb in T-cell development. Genes Dev. 8(7): 770-782. Medline abstract: 95011579 Bin, X. and Lipsick, J. S. (1993). Individual repeats of Drosophila Myb can function in transformation by v-Myb. J. Virol. 67(12): 7332-7339. Medline abstract: 94047348 Coffman, J. A., et al. (1997). SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin embryos. Development 124(23): 4717-4727. Medline abstract: 98088684 DeRocco, S. E., et al. (1997). Ectopic expression of A-myb in transgenic mice causes follicular hyperplasia and enhanced B lymphocyte proliferation. Proc. Natl. Acad. Sci. 94(7): 3240-3244. Medline abstract: 97250521 Ess, K. C., et al. (1995). A central role for a single c-Myb binding site in a thymic locus control region. Mol. Cell. Biol. 15(10): 5707-5715. Medline abstract: 96009598 Facchinetti, V., et al. (1997). Regulatory domains of the A-Myb transcription factor and its interaction with the CBP/p300 adaptor molecules. Biochem J. 324(Pt 3): 729-736. Medline abstract: 97327718 Frampton, J., Ramqvist, T., Graf, T. (1996). v-Myb of E26 leukemia virus up-regulates bcl-2 and suppresses apoptosis in myeloid cells. Genes Dev 10(21): 2720-2731. Medline abstract: 97102673 Fung, S. M., Ramsay, G. and Katzen, A. L. (2002). Mutations in Drosophila myb lead to centrosome amplification and genomic instability. Development 129: 347-359. Medline abstract: 11807028 Golay, J., et al. (1997). Redundant functions of B-Myb and c-Myb in differentiating myeloid cells. Cell Growth Differ. 8(12): 1305-1316. Medline abstract: 98080689 Guo, K., et al. (1999). A myb-related protein required for culmination in Dictyostelium. Development 126(12): 2813-2822 Hedge, S. P., et al. (1998). c-Maf interacts with c-Myb to regulate transcription of an early myeloid gene during differentiation. Mol. Cell. Biol. 18(5): 2729-2737. Medline abstract: 98226545 Hou, D. X., Akimaru, H. and Ishii, S. (1997). Trans-activation by the Drosophila myb gene product requires a Drosophila homologue of CBP. FEBS Lett. 413(1): 60-64. Medline abstract: 97431612 Katzen, A. L., Kornberg, T. B. and Bishop, J. M. (1985). Isolation of the proto-oncogene c-myb from D. melanogaster. Cell 41(2): 449-456. Medline abstract: 85176969 Katzen, A. L. and Bishop, J. M. (1996). myb provides an essential function during Drosophila development. Proc. Natl. Acad. Sci. 93(24): 13955-13960. Medline abstract: 97098502 Katzen, K. L., et al. (1998). Drosophila myb is required for the G2/M transition and maintenance of diploidy. Genes Dev. 12: 831-843. Medline abstract: 98180985 Kowenz-Leutz, E., et al. (1997). The homeobox gene GBX2, a target of the myb oncogene, mediates autocrine growth and monocyte differentiation. Cell 91(2): 185-195. Medline abstract: 98004222 Ku, D. H., et al. (1993). c-myb transactivates cdc2 expression via Myb binding sites in the 5'-flanking region of the human cdc2 gene. J. Biol. Chem. 268(3): 2255-2259. Medline abstract: 93131991 Lam, E. W., Bennett, J. D. and Watson, R. J. (1995). Cell-cycle regulation of human B-myb transcription. Gene 160(2): 277-281. Medline abstract: 95369704 Liu, N., et al. (1996). Cell cycle-regulated repression of B-myb transcription: cooperation of an E2F site with a contiguous corepressor element. Nucleic Acids Res. 24(15): 2905-2910. Medline abstract: 96357121 Lucibello, F. C., et al. (1997). The differential binding of E2F and CDF repressor complexes contributes to the timing of cell cycle-regulated transcription. Nucleic Acids Res. 25(24): 4921-4925. Medline abstract: 98060887 Ly, D. H., Lockhart, D. J., Lerner, R. A. and Schultz, P. G. (2000). Mitotic misregulation and human aging. Science 287: 2486-92. Medline abstract: 10741968 Madan, A., et al. (1995). Bacterial expression, characterization and DNA binding studies on Drosophila melanogaster c-Myb DNA-binding protein. Eur. J. Biochem. 232(1): 150-158. Medline abstract: 96048041 Miglarese, M. R., Halaban, R. and Gibson, N. W. (1997). Regulation of fibroblast growth factor 2 expression in melanoma cells by the c-MYB proto-oncoprotein. Cell Growth Differ. 8(11): 1199-1210. Medline abstract: 98039512 Oelgeschlager, M., et al. (1996). C/EBP, c-Myb, and PU.1 cooperate to regulate the neutrophil elastase promoter. Mol. Cell. Biol. 16(9): 4717-4725. Medline abstract: 96347540 Oh, I. H. and Reddy, E. P. (1997). Murine A-myb gene encodes a transcription factor, which cooperates with Ets-2 and exhibits distinctive biochemical and biological activities from c-myb. J. Biol. Chem. 272(34): 21432-21443. Medline abstract: 97407935 Oh, I. H. and Reddy, E. P. (1998). The C-terminal domain of B-Myb acts as a positive regulator of transcription and modulates its biological functions. Mol. Cell. Biol. 18(1): 499-511. Medline abstract: 98078706 Okada, M., et al. (2002). Myb controls G2/M progression by inducing cyclin B expression in the Drosophila eye imaginal disc. EMBO J. 21: 675-684. Medline abstract: 11847115 Otsuka, H. and Van Haastert, P. J. M. (1998). A novel Myb homolog initiates Dictyostelium development by induction of adenylyl cyclase expression. Genes Dev. 12: 1738-1748. Medline abstract: 98283920 Peters, C. W., et al. (1987). Drosophila and vertebrate myb proteins share two conserved regions, one of which functions as a DNA-binding domain. EMBO J. 6(10): 3085-3090. Medline abstract: 88082681 Postigo, A. A., et al. (1997). c-Myb and Ets proteins synergize to overcome transcriptional repression by ZEB. EMBO J. 16(13): 3924-3934. Medline abstract: 97377031 Raschella, G., et al. (1997). Retinoblastoma-related protein pRb2/p130 and its binding to the B-myb promoter increase during human neuroblastoma differentiation. J. Cell Biochem. 67(3): 297-303. Medline abstract: 98027077 Robinson, C., et al. (1996). Cell-cycle regulation of B-Myb protein expression: specific phosphorylation during the S phase of the cell cycle. Oncogene 12(9): 1855-1864. Medline abstract: 96211391 Sala, A. and Calabretta, B. (1992). Regulation of BALB/c 3T3 fibroblast proliferation by B-myb is accompanied by selective activation of cdc2 and cyclin D1 expression. Proc. Natl. Acad. Sci. 89(21): 10415-10419. Medline abstract: 93066249 Sala, A., et al. (1996). B-myb promotes S phase and is a downstream target of the negative regulator p107 in human cells. J. Biol. Chem. 271(16): 9363-9367. Medline abstract: 96199187 Sala, A., et al. (1997). Activation of human B-MYB by cyclins. Proc. Natl. Acad. Sci. 94(2): 532-536. Medline abstract: 97165056 Schaefer, A., et al. (1996). Ca2+/calmodulin-dependent and -independent down-regulation of c-myb mRNA levels in erythropoietin-responsive murine erythroleukemia cells. The role of calcineurin. J. Biol. Chem. 271(23): 13484-13490. Medline abstract: 96278771 Sitzmann, J., et al. (1995). Expression of mouse c-myb during embryonic development. Oncogene 11(11): 2273-2279. Medline abstract: 96112628 Sitzmann, J., et al. (1996). Expression of B-Myb during mouse embryogenesis. Oncogene 12(9): 1889-1894. Medline abstract: 96211395 Suhasini, M., et al. (1998). cAMP-induced NF-kappaB (p50/relB) binding to a c-myb intronic enhancer correlates with c-myb up-regulation and inhibition of erythroleukemia cell differentiation. Oncogene 15(15): 1859-1870. Medline abstract: 98026826 Tahirov, T. H., et al. (2002). Mechanism of c-Myb-C/EBPß cooperation from separated sites on a promoter. Cell 108: 57-70. Medline abstract: 11792321 Taylor, D., Badiani, P. and Weston, K. (1996). A dominant interfering Myb mutant causes apoptosis in T cells. Genes Dev. 10(21): 2732-2744. Medline abstract: 97102674 Toscani, A., et al. (1997). Arrest of spermatogenesis and defective breast development in mice lacking A-myb. Nature 386(6626): 713-717. Medline abstract: 97263572 Trauth, K., et al. (1994). Mouse A-myb encodes a trans-activator and is expressed in mitotically active cells of the developing central nervous system, adult testis and B lymphocytes. EMBO J. 13(24): 5994-6005. Medline abstract: 95112810 Ying, G. G., et al. (1997). The DNA binding domain of the A-MYB transcription factor is responsible for its B cell-specific activity and binds to a B cell 110-kDa nuclear protein. J. Biol. Chem. 272(40): 24921-24926. Medline abstract: 97460074 Ziebold, U., and Klempnauer, K. H. (1997). Linking Myb to the cell cycle: cyclin-dependent phosphorylation and regulation of A-Myb activity. Oncogene 15(9): 1011-1019. Medline abstract: 97429917
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,341 Da
NCBI Official Full Name
transcriptional activator Myb isoform 3
NCBI Official Synonym Full Names
v-myb myeloblastosis viral oncogene homolog (avian)
NCBI Official Symbol
MYB
NCBI Official Synonym Symbols
efg; Cmyb; c-myb; c-myb_CDS
NCBI Protein Information
transcriptional activator Myb; c-myb8B_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; proto-oncogene c-Myb; c-myb protein (140 AA)
UniProt Protein Name
Transcriptional activator Myb
Protein Family
UniProt Gene Name
MYB
UniProt Entry Name
MYB_HUMAN
NCBI Description
This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]
Uniprot Description
Myb: Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Oncoprotein; DNA-binding
Chromosomal Location of Human Ortholog: 6q22-q23
Cellular Component: nuclear matrix; cytosol
Molecular Function: protein binding; chromatin binding
Biological Process: positive regulation of histone H3-K9 methylation; thymus development; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; homeostasis of number of cells; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; chromatin remodeling; positive regulation of T-helper cell differentiation; regulation of transcription, DNA-dependent; calcium ion transport; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; blood coagulation; embryonic gut development; G1/S transition of mitotic cell cycle
