Mouse anti-Human Mucin 5B Monoclonal Antibody | anti-MUC5B antibody

Mucin 5B (MUC5B)

Cat. Num. AAA601729

• Gene Names: MUC5B; MG1; MUC5; MUC9; MUC-5B
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Mucin 5B; Monoclonal Antibody; Mucin 5B (MUC5B); Anti -Mucin 5B (MUC5B); anti-MUC5B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4H310
• Specificity: Recognizes the calculated ~590kD human MUC5B protein. On Western Blots, recognizes a band significantly higher than 250kD. Reactivity has been confirmed with deglycosylated and secreted media from primary human tracheobranchial epithelial culture and a deglycosylated human saliva sample. Immunohistochemical assays were also performed using trachea samples from both normal and chronic obstructive pulmonary disease patients.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-MUC5B antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 0.1-1ug/ml; Western Blot: 5-10ug/ml; Immunohistochemistry (FFPE): 5-10ug/ml; Note: Western Blot and Immunohistochemistry require deglycosylation. Refer to protocols.
• Immunogen: Synthetic peptide derived from the N-terminal region of the human MUC5B protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-MUC5B antibody

Mucins (MUCs) are heavily glycosylated glycoproteins that are synthesized either as membrane-bound or secreted proteins. Mucins are noteworthy because they have an aberrant expression profile in various malignancies. Mucins normally play a protective role for epithelial tissues. They are expressed in the gastrointestinal, respiratory and genito-urinary tracts. Mucins may be involved in the renewal and differentiation of the epithelium, and the modulation of cell adhesion and cell signaling. MUC5B is thought to play a vital role in maintaining oral health, and may be involved in respiratory and oral disease. MUC5B expression has been studied in gastric carcinoma as well as chronic obstructive pulmonary disease.

Product Categories/Family for anti-MUC5B antibody

Antibodies; Abs to Mucin

NCBI and Uniprot Product Information

• NCBI GI #: 153945878
• NCBI GeneID: 727897
• NCBI Accession #: NP_002449.1
• NCBI GenBank Nucleotide #: NM_002458.1
• UniProt Accession #: O00446; Q14879; Q14880; Q14882; Q93043; Q9HC84; Q9NYE4
• Molecular Weight: 32,064 Da
• NCBI Official Full Name: mucin-5B
• NCBI Official Synonym Full Names: mucin 5B, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC5B
• NCBI Official Synonym Symbols: MG1; MUC5; MUC9; MUC-5B
• NCBI Protein Information: mucin-5B; OTTHUMP00000233052; cervical mucin MUC5B; sublingual gland mucin; mucin-5 subtype B, tracheobronchial; mucin 5, subtype B, tracheobronchial; high molecular weight salivary mucin MG1
• UniProt Protein Name: Mucin MUC5B
• Protein Family: Mucin
• UniProt Entry Name: O00446_HUMAN

NCBI Description

This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq]

Uniprot Description

MUC5B: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. Defects in MUC5B are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. A common polymorphism in the promoter of MUC5B is associated with familial interstitial pneumonia and idiopathic pulmonary fibrosis, suggesting that dysregulated MUC5B expression in the lung may be involved in the pathogenesis of pulmonary fibrosis (PubMed:21506741).

Protein type: Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; regulation of macrophage activation; defense response to bacterium; post-translational protein modification

Disease: Pulmonary Fibrosis, Idiopathic