Western Blot (WB)
(CAF-1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).)
Rabbit CAF-1 Polyclonal Antibody | anti-CNOT7 antibody
CAF-1 Antibody (N-term)
Western Blot (WB)
(CAF-1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).)
Western Blot (WB)
(CAF-1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).)
Immunohistochemistry (IHC)
(CAF-1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CAF-1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
anti-proliferative protein, B-cell translocation protein 1, which
negatively regulates cell proliferation. Binding of the two
proteins, which is driven by phosphorylation of the
anti-proliferative protein, causes signaling events in cell
division that lead to changes in cell proliferation associated with
cell-cell contact. The protein has both mouse and yeast orthologs.
Alternate splicing of this gene results in two transcript variants
encoding different isoforms.
Aslam, A., et al. Mol. Biol. Cell 20(17):3840-3850(2009)
Miyasaka, T., et al. Cancer Sci. 99(4):755-761(2008)
Nishida, K., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63 (PT 12), 1061-1063 (2007) :
Robin-Lespinasse, Y., et al. J. Cell. Sci. 120 (PT 4), 638-647 (2007) :
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
Uniprot Description
CNOT7: Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression. Belongs to the CAF1 family.
Protein type: Transcription, coactivator/corepressor; EC 3.1.13.4
Chromosomal Location of Human Ortholog: 8p22-p21.3
Cellular Component: membrane; nucleus; cytosol; CCR4-NOT complex
Molecular Function: protein binding; signal transducer activity; 3'-5'-exoribonuclease activity; poly(A)-specific ribonuclease activity; exoribonuclease activity; RNA binding; metal ion binding; transcription factor activity
Biological Process: deadenylation-dependent decapping; transcription, DNA-dependent; miRNA-mediated gene silencing; signal transduction; regulation of translation; negative regulation of cell proliferation; poly(A) tail shortening; carbohydrate metabolic process; positive regulation of cell proliferation; cytoplasmic mRNA processing body assembly; gene expression; positive regulation of transcription from RNA polymerase II promoter; RNA-mediated gene silencing; mRNA catabolic process, deadenylation-dependent decay
Research Articles on CNOT7
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Product Notes
The CNOT7 cnot7 (Catalog #AAA9202707) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 34-61. The CAF-1 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Chicken, Zebrafish, Mouse, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's CAF-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the CNOT7 cnot7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAF-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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