Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Monoclonal Antibody (CLH5).)
Mouse anti-Human MUC6 (Mucin 6 / Gastric Mucin) Monoclonal Antibody | anti-MUC6 antibody
MUC6 (Mucin 6 / Gastric Mucin) Mouse Monoclonal Antibody
Gene Names
MUC6; MUC-6
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
MUC6 (Mucin 6 / Gastric Mucin); Monoclonal Antibody; MUC6 (Mucin 6 / Gastric Mucin) Mouse Monoclonal Antibody; Gastric mucin 6; MUC6; MUC6 mucin; Mucin 6 oligomeric mucus/gel forming; Mucin glycoprotein Fragment; Mucin-6; Secretory mucin MUC6; anti-MUC6 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
CLH5
Specificity
The MUC6 gastric mucin is a secreted glycoprotein that plays an essential role in epithelial cyto-protection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 is associated with Lewis type 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
2439
Applicable Applications for anti-MUC6 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Cytoplasmic & Secreted
Immunogen
A synthetic peptide of the Gastric Mucin 6 tandem repeat sequence
Hu-Chromosome Location
11p15.5
Positive Control
Stomach
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Monoclonal Antibody (CLH5).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Monoclonal Antibody (CLH5).)
References
Reis C A, David L, Correa P, et al. Intestinal metaplasia of human stomach displays distinct patterns of mucin (MUC1, MUC2, MUC5AC, and MUC6) expression. Cancer Res. 59(5): 1003-1007 (1999
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
252kDa
NCBI Official Full Name
mucin-6
NCBI Official Synonym Full Names
mucin 6, oligomeric mucus/gel-forming
NCBI Official Symbol
MUC6
NCBI Official Synonym Symbols
MUC-6
NCBI Protein Information
mucin-6
UniProt Protein Name
Mucin-6
Protein Family
UniProt Gene Name
MUC6
UniProt Synonym Gene Names
MUC-6
UniProt Entry Name
MUC6_HUMAN
NCBI Description
The MUC6 gene encodes gastric mucin, a secreted glycoprotein that plays an essential role in epithelial cytoprotection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract (summary by Toribara et al., 1993 [PubMed 7680650]).[supplied by OMIM, Dec 2010]
Uniprot Description
MUC6: May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: Golgi lumen
Biological Process: O-glycan processing
Research Articles on MUC6
Similar Products
Product Notes
The MUC6 muc6 (Catalog #AAA439358) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MUC6 (Mucin 6 / Gastric Mucin) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MUC6 (Mucin 6 / Gastric Mucin) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the MUC6 muc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC6 (Mucin 6 / Gastric Mucin), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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