Rabbit anti-Human MUC5AC/Mucin 5Ac Monoclonal Antibody | anti-MUC5AC antibody

Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody

Cat. Num. AAA179390

• Gene Names: MUC5AC; TBM; leB; MUC5; mucin
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Affinity-chromatography
• Synonyms: MUC5AC/Mucin 5Ac; Monoclonal Antibody; Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody; Mucin-5AC; MUC-5AC; Gastric mucin; Lewis B blood group antigen; LeB; Major airway glycoprotein; Mucin-5 subtype AC; tracheobronchial; Tracheobronchial mucin; TBM; MUC5AC; MUC5; anti-MUC5AC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Purity/Purification: Affinity-chromatography
• Form/Format: Liquid
• Sequence Length: 5,654

• Applicable Applications for anti-MUC5AC antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC/IF: 1:50-1:200
• Immunogen: A synthesized peptide derived from human MUC5AC
• Subcellular Localization: Secreted.
• Tissue Specificity: Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
• Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of MUC5AC expression in human stomach lysate (MBS179390).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC5AC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MUC5AC )

Related Product Information for anti-MUC5AC antibody

Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.

Product Categories/Family for anti-MUC5AC antibody

Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Proteins; muc; Cancer; Invasion/Microenvironment; Tumor Immunology; tumor-associated antigens; Tumor Biomarkers; Tumor Antigens

NCBI and Uniprot Product Information

• NCBI GI #: 748983076
• NCBI GeneID: 4586
• NCBI Accession #: NP_001291288.1
• NCBI GenBank Nucleotide #: NM_001304359.1
• UniProt Accession #: P98088; O60460; O76065; Q13792; Q14425; Q658Q1; Q7M4S5; Q8N4M9; Q8WWQ3; Q8WWQ4; Q8WWQ5; A0A096LPK4
• Molecular Weight: 585570 MW
• NCBI Official Full Name: mucin-5AC
• NCBI Official Synonym Full Names: mucin 5AC, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC5AC
• NCBI Official Synonym Symbols: TBM; leB; MUC5; mucin
• NCBI Protein Information: mucin-5AC
• UniProt Protein Name: Mucin-5AC
• Protein Family: Mucin
• UniProt Gene Name: MUC5AC
• UniProt Synonym Gene Names: TBM

Uniprot Description

Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.