Mouse anti-Human MUC2 (Mucin 2) Monoclonal Antibody | anti-MUC2 antibody

MUC2 (Mucin 2) Mouse Monoclonal Antibody

Cat. Num. AAA438360

• Gene Names: MUC2; MLP; SMUC; MUC-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: MUC2 (Mucin 2); Monoclonal Antibody; MUC2 (Mucin 2) Mouse Monoclonal Antibody; Mucin-2; Intestinal mucin-2; Mucin like protein; (MLP); SMUC; anti-MUC2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CCP58
• Specificity: Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro). Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 5179

• Applicable Applications for anti-MUC2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic and Cell Surface
• Immunogen: A synthetic peptide of 29 amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH.
• Hu-Chromosome Location: 11p15.5
• Positive Control: LS174T cells or small intestine.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Intestine stained with MUC2 Monoclonal Antibody (CCP58).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC2 Monoclonal Antibody (CCP58).)

References

Xing PX, Prenzoska J, Layton GT, Devine PL, McKenzie IF. Second-generation monoclonal antibodies to intestinal MUC2 peptide reactive with colon cancer. J Natl Cancer Inst. 1992; 84(9):699-703

NCBI and Uniprot Product Information

• NCBI GI #: 557129045
• NCBI GeneID: 4583
• NCBI Accession #: NP_002448.3
• NCBI GenBank Nucleotide #: NM_002457.3
• UniProt Accession #: Q02817; Q14878
• Molecular Weight: 520kDa
• NCBI Official Full Name: mucin-2
• NCBI Official Synonym Full Names: mucin 2, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC2
• NCBI Official Synonym Symbols: MLP; SMUC; MUC-2
• NCBI Protein Information: mucin-2
• UniProt Protein Name: Mucin-2
• Protein Family: Mucin
• UniProt Gene Name: MUC2
• UniProt Synonym Gene Names: SMUC; MUC-2
• UniProt Entry Name: MUC2_HUMAN

NCBI Description

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

MUC2: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: Golgi lumen

Molecular Function: protein binding

Biological Process: maintenance of gastrointestinal epithelium; O-glycan processing