Mononucleosomes H3K14ac Recombinant Protein | H3K14ac recombinant protein

Recombinant Mononucleosomes H3K14ac (EPL)-biotin

Cat. Num. AAA389063

• Gene Names: HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
• Purity: The recombinant protein is >=95% by SDS-PAGE gel.
• Synonyms: Mononucleosomes H3K14ac; Recombinant Mononucleosomes H3K14ac (EPL)-biotin; H3K14ac recombinant protein

• Host: E Coli
• Purity/Purification: The recombinant protein is >=95% by SDS-PAGE gel.
• Form/Format: Recombinant Mononucleosomes H3K14ac (EPL)-biotinylated (20ug protein + 20ug DNA) is supplied at a protein concentration of 0.45ug/ul in 10mM Tris-HCl, pH8.0, 1mM EDTA, 2mM DTT and 20% glycerol.

• Protein Species: Human
• Conjugation: Acetylated, Biotinylated
• Notes: Recombinant Mononucleosomes H3K14ac (EPL)-biotinylated are suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Streptavidin pull-down for Recombinant Mononucleosomes H3K14ac (EPL) - biotin. Recombinant Mononucleosomes H3K14ac (EPL) were pulled down by streptavidin beads. Input nucleosomes (Lane 2) and the nucleosomes (Lane 3) pulled down by streptavidin were run on a 13% SDS-PAGE gel and stained with Commassie blue. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes H3K14ac are pulled down by streptavidin beads. * indicates streptavidin.)

Testing Data

(Recombinant Mononucleosomes H3K14ac (EPL) - biotin, DNA gel. Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H3K14ac. Intact mononucleosomes H3K14ac migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)

Western Blot (WB)

(Western Blot analysis for Recombinant Mononucleosomes H3K14ac (EPL) - biotin. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated (Lane 2) were detected with anti-H3K14ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K14ac (EPL) - biotinylated can be detected by anti-H3K14ac antibody.)

Related Product Information for H3K14ac recombinant protein

Short Description: Recombinant Mononucleosomes H3K14ac (EPL)-biotinylated consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NP_003503.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end)(accession number NP_066403.2) with acetylation at lysine 14, and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli. The molecular weight of histone octamer is ~108 kDa. The recombinant protein is >=95% by SDS-PAGE gel. H3K14ac (Histone H3 acetyl Lys14) protein is generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E Coli and purified using FPLC. The purified protein is subsequently ligated to an N-terminal histone tail peptide containing acetyl lysine 14 via a native peptide bond.

Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Product Categories/Family for H3K14ac recombinant protein

Recombinant Proteins; Histone

NCBI and Uniprot Product Information

• NCBI GI #: 4504245
• NCBI GeneID: 8334
• NCBI Accession #: NP_003503.1; NP_003509.1; NP_066403.2; NP_003539.1
• NCBI GenBank Nucleotide #: NM_003512.3
• UniProt Accession #: Q93077; O00775; O00776; O00777; O00778; Q540R1; B2R4F7
• Molecular Weight: 14,105 Da
• NCBI Official Full Name: histone H2A type 1-C
• NCBI Official Synonym Full Names: histone cluster 1 H2A family member c
• NCBI Official Symbol: HIST1H2AC
• NCBI Official Synonym Symbols: H2A/l; H2AFL; dJ221C16.4
• NCBI Protein Information: histone H2A type 1-C
• UniProt Protein Name: Histone H2A type 1-C
• UniProt Gene Name: HIST1H2AC
• UniProt Synonym Gene Names: H2AFL

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.