Immunofluorescence (IF)
(Confocal Immunofluorescent Immunocytochemistry on HeLa cells. mTOR is in red using M4696-10D.)
Mouse anti-Human mTOR Monoclonal Antibody | anti-mTOR antibody
mTOR (Mammalian target of rapamycin, mechanistic target of rapamycin (serine/threonine kinase), FK506-binding Protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, Rapamycin Ta
Immunofluorescence (IF)
(Confocal Immunofluorescent Immunocytochemistry on HeLa cells. mTOR is in red using M4696-10D.)
Immunofluorescence (IF)
(Confocal Immunofluorescent Immunocytochemistry on HeLa cells. mTOR is in red using M4696-10D.)
Immunocytochemistry (ICC)
(Confocal Immunofluorescent Immunocytochemistry Representative lot data using M4696-10D HeLa cells (top) and A431 cells (bottom) were triple stained with M4696-10D (Red, Cy3) (1:500), Actin (Phalloidin, Green), and DAPI (nuclei, blue). The figures on the left are with the green 488 filter off.)
Immunocytochemistry (ICC)
(Confocal Immunofluorescent Immunocytochemistry Representative lot data using M4696-10D HeLa cells (top) and A431 cells (bottom) were triple stained with M4696-10D (Red, Cy3) (1:500), Actin (Phalloidin, Green), and DAPI (nuclei, blue). The figures on the left are with the green 488 filter off.)
SDS-Page
(Representative lot data using M4696-10D HEK293 cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane, and probed with M4696-10D (1:2000). Proteins were visualized using an IgG (HRP) donkey anti-mouse secondary antibody and a chemiluminescence detection system. Arrow indicates mTOR (289kD).)
SDS-Page
(Representative lot data using M4696-10D HEK293 cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane, and probed with M4696-10D (1:2000). Proteins were visualized using an IgG (HRP) donkey anti-mouse secondary antibody and a chemiluminescence detection system. Arrow indicates mTOR (289kD).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
Uniprot Description
RICTOR: a regulatory binding partner of the kinase mTOR. Part of a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Rictor interacts with Cullin1-Rbx1 to form an E3 ubiquitin ligase complex, and promotes ubiquitination and degradation of SGK1. Shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.
Protein type: Cell cycle regulation; Ubiquitin conjugating system; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 5p13.1
Cellular Component: TORC2 complex; cytosol
Molecular Function: protein binding; ribosome binding; protein kinase binding
Biological Process: epidermal growth factor receptor signaling pathway; TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; actin cytoskeleton reorganization; innate immune response; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway
Research Articles on mTOR
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Product Notes
The mTOR rictor (Catalog #AAA6246189) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The mTOR (Mammalian target of rapamycin, mechanistic target of rapamycin (serine/threonine kinase), FK506-binding Protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, Rapamycin Ta reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's mTOR can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). Researchers should empirically determine the suitability of the mTOR rictor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mTOR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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