SDS-Page
Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 4 (PIN4) Recombinant Protein | PIN4 recombinant protein
Recombinant Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 4 (PIN4)
Gene Names
PIN4; EPVH; PAR14; PAR17
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 4 (PIN4); Recombinant Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 4 (PIN4); PIN4 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- SADKKAQG PKGGGNAVKV RHILCEKHGK IMEAMEKLKS GMRFNEVAAQ YSEDKARQGG DLGWMTRGSM VGPFQEAAFA LPVSGMDKPV FTDPPVKTKF GYHIIMVEGR
MGHHHHHHSGSEF- SADKKAQG PKGGGNAVKV RHILCEKHGK IMEAMEKLKS GMRFNEVAAQ YSEDKARQGG DLGWMTRGSM VGPFQEAAFA LPVSGMDKPV FTDPPVKTKF GYHIIMVEGR
Sequence Length
133
Applicable Applications for PIN4 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Ser23~Arg130 (Accession # Q9Y237) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13.3kDa
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isoform 2
NCBI Official Synonym Full Names
protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
NCBI Official Symbol
PIN4
NCBI Official Synonym Symbols
EPVH; PAR14; PAR17
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase NIMA-interacting 4; hEPVH; hPar14; hPar17; PPIase PIN4; parvulin-14; parvulin-17; rotamase PIN4; eukaryotic parvulin homolog; peptidyl-prolyl cis-trans isomerase Pin4; peptidyl-prolyl cis/trans isomerase EPVH
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
Protein Family
UniProt Gene Name
PIN4
UniProt Synonym Gene Names
Par14; hPar14; Par17; hPar17; PPIase Pin4; hEPVH
UniProt Entry Name
PIN4_HUMAN
NCBI Description
This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009]
Uniprot Description
PIN4: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily. 2 isoforms of the human protein are produced by alternative promoter.
Protein type: EC 5.2.1.8; Mitochondrial; Isomerase; Nucleolus
Chromosomal Location of Human Ortholog: Xq13
Cellular Component: mitochondrial matrix; cytoplasm; nucleolus; spindle; nucleus
Molecular Function: peptidyl-prolyl cis-trans isomerase activity; DNA binding; double-stranded DNA binding; bent DNA binding
Biological Process: protein peptidyl-prolyl isomerization; rRNA processing
Research Articles on PIN4
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Product Notes
The PIN4 pin4 (Catalog #AAA2012450) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 4 (PIN4) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the PIN4 pin4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF- SADKKAQG PKGGGNAVKV RHILCEKHGK IMEAMEKLKS GMRFNEVAAQ YSEDKARQGG DLGWMTRGSM VGPFQEAAFA LPVSGMDKPV FTDPPVKTKF GYHIIMVEGR. It is sometimes possible for the material contained within the vial of "Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 4 (PIN4), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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