Mouse anti-Human, Mouse MTHFD2 Monoclonal Antibody | anti-MTHFD2 antibody

MTHFD2 Antibody (C-term)

Cat. Num. AAA9231324

• Gene Names: MTHFD2; NMDMC
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified through a protein G column, followed by dialysis against PBS.
• Synonyms: MTHFD2; Monoclonal Antibody; MTHFD2 Antibody (C-term); Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase; mitochondrial; NAD-dependent methylenetetrahydrofolate dehydrogenase; 1.5.1.15; Methenyltetrahydrofolate cyclohydrolase; 3.5.4.9; NMDMC; anti-MTHFD2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 1985CT220.118.31
• Specificity: This MTHFD2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 266-299 amino acids from the C-terminal region of human MTHFD2.
• Purity/Purification: Purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 350

• Applicable Applications for anti-MTHFD2 antibody: Western Blot (WB), ELISA (EIA)
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Dilution: WB - 1:4000All lanes: Anti-MTHFD2 Antibody (C-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: Mouse heart lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-MTHFD2 antibody

Signal Transduction

References

Peri K.G.,et al.Nucleic Acids Res. 17:8853-8853(1989). Ota T.,et al.Nat. Genet. 36:40-45(2004). Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Hillier L.W.,et al.Nature 434:724-731(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 94721354
• NCBI GeneID: 10797
• NCBI Accession #: NP_006627.2
• NCBI GenBank Nucleotide #: NM_006636.4
• UniProt Accession #: P13995
• Molecular Weight: 37895 Da
• NCBI Official Full Name: bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• NCBI Official Symbol: MTHFD2
• NCBI Official Synonym Symbols: NMDMC
• NCBI Protein Information: bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
• UniProt Protein Name: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
• Protein Family: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase
• UniProt Gene Name: MTHFD2
• UniProt Synonym Gene Names: NMDMC
• UniProt Entry Name: MTDC_HUMAN

NCBI Description

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Uniprot Description

MTHFD2: a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 1.5.1.15; EC 3.5.4.9; Hydrolase; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: cytosol; extracellular space; mitochondrial matrix; mitochondrion

Molecular Function: formate-tetrahydrofolate ligase activity; magnesium ion binding; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; phosphate binding

Biological Process: folic acid metabolic process; one-carbon compound metabolic process; tetrahydrofolate metabolic process