Mouse anti-Human, Rat Msx2 Monoclonal Antibody | anti-MSX2 antibody

Msx2 (Hox8)

Cat. Num. AAA600152

• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Msx2; Monoclonal Antibody; Msx2 (Hox8); Anti -Msx2 (Hox8); anti-MSX2 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 4E158
• Specificity: Recognizes an ~30kD protein, corresponding to the apparent molecular mass of human Msx2 at ~30kD. Species crossreactivity: rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 40% glycerol.

• Applicable Applications for anti-MSX2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot (ECL): 1ug/ml; Immunohistochemistry: 1-10ug/ml; Optimal dilutions to be determined by researcher.
• Immunogen: Recombinant human Msx2 protein (corresponding to the N-terminal region, aa 1-77).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of HeLa (1), ZR-75-1 (2), KB (3) cell lysates, and recombinant Msx2 protein (4), probed with MBS600152)

Related Product Information for anti-MSX2 antibody

Msx2, also known as HOX-8, is a homeobox containing transcription factor which is a member of the MSH family of homeobox proteins. Homeobox genes (HOX genes), encode DNA binding proteins that recognize specific sequences and modulate transcriptional activity (1). Msx-2 is believed to play a role in regulating bone development, particularly in sutures of cranial bone (5). Msx-2 is also expressed in sites of skin appendage formation and it has been proposed that Msx-2 is involved in the growth of skin and skin appendages (6). Msx2 mRNA is found expressed at higher levels in immature tumors, including a yolk sac tumor, seminoma, and chroiocarcinoma, and also in various tumors of epithelial origin (2). Msx2 gene expression is frequently activated in carcinoma-derived cell lines. The gene is inactive in NIH3T3 cells but is transcriptionally activated after transformation by v-Ki-ras suggesting that the Msx2 may play a role in cell transformation (3). Msx2 defects are a cause of craniosynostosis type 2 (CRS2), a disease that is characterized by premature fusion of calvarial sutures that leads to abnormal skull shape (4).

Product Categories/Family for anti-MSX2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 124111252
• NCBI GeneID: 744194
• UniProt Accession #: A2T764
• Molecular Weight: 28,921 Da
• NCBI Official Full Name: MSX2
• NCBI Official Symbol: MSX2
• NCBI Protein Information: homeobox protein MSX-2
• UniProt Protein Name: Homeobox protein MSX-2
• Protein Family: Msx2-interacting protein
• UniProt Gene Name: MSX2
• UniProt Entry Name: MSX2_PANTR

Uniprot Description

Function: Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter

Subcellular location: Nucleus

Sequence similarities: Belongs to the Msh homeobox family.Contains 1 homeobox DNA-binding domain.