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Western Blot (WB)
(Western Blot analysis using MBS602417 (2ug/ml):|Lane 1: Non-treated HeLa cell lysates |Lane 2: colcemid-treated HeLa cell lysates)
Mouse anti-Human MPM2, Ser/Thr, Pro Monoclonal Antibody
MPM2, Ser/Thr, Pro, phosphorylated (Mitotic Protein Monoclonal#2)
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
MPM2; Ser/Thr; Pro; Monoclonal Antibody; phosphorylated (Mitotic Protein Monoclonal#2); Anti -MPM2; anti-MPM2; Pro antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
0.T.181
Specificity
Recognizes human MPM2. Reacts with phosphorylated serines or threonines when followed by a proline. Recognizes a phosphorylated epitope (S/T)P found in phosphoproteins such as MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM2 varies from species to species and with the cell type. Species Crossreactivity: animals and plants.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Applicable Applications for anti-MPM2, Ser/Thr, Pro antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunocytochemistry, ELISA, Immunohistochemistry, Immunoprecipitation and Flow Cytometry.
Dilution: Western Blot: 0.5-2ug/ml detects mitosis-associated proteins in colcemid treated (0.05ug/ml) human HeLa carcinoma cells. Colcemid-treated HeLa cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with M4685. Proteins were visualized using an IgG (HRP) goat anti-mouse antibody and ECL.
Immunocytochemistry: 5-15ug/ml shows positive staining of mitotic cells.
ELISA: Used in a non-radioactive, dissociation-enhanced time resolved fluoroimmunoassay (DELPHIA). Active enzymes were used to phosphorylate substrate peptides. The phosphorylated peptides were detected using MPM2 in conjunction with a europium (Eu3+) labeled secondary antibody. The phosphorylated peptides detected include: Serine/Threonine Kinase Substrate Peptide, Recombinant, Human (Biotin).
Immunoprecipitation: Reported by an independent laboratory.
Dilution: Western Blot: 0.5-2ug/ml detects mitosis-associated proteins in colcemid treated (0.05ug/ml) human HeLa carcinoma cells. Colcemid-treated HeLa cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with M4685. Proteins were visualized using an IgG (HRP) goat anti-mouse antibody and ECL.
Immunocytochemistry: 5-15ug/ml shows positive staining of mitotic cells.
ELISA: Used in a non-radioactive, dissociation-enhanced time resolved fluoroimmunoassay (DELPHIA). Active enzymes were used to phosphorylate substrate peptides. The phosphorylated peptides were detected using MPM2 in conjunction with a europium (Eu3+) labeled secondary antibody. The phosphorylated peptides detected include: Serine/Threonine Kinase Substrate Peptide, Recombinant, Human (Biotin).
Immunoprecipitation: Reported by an independent laboratory.
Immunogen
Mitotic human HeLa cell cytosolic lysate.
Positive Control
Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis using MBS602417 (2ug/ml):|Lane 1: Non-treated HeLa cell lysates |Lane 2: colcemid-treated HeLa cell lysates)
Western Blot (WB)
(Western Blot analysis using MBS602417 (2ug/ml):|Lane 1: Non-treated HeLa cell lysates |Lane 2: colcemid-treated HeLa cell lysates)
Immunostaining (IS)
(MPM-2 immunostaining (green) of phospho-proteins in metaphase cells using MBS602417. The spindle, centrosome and centromeric regions are stained. DNA is counterstained with propidium iodide (red).)
Immunostaining (IS)
(MPM-2 immunostaining (green) of phospho-proteins in metaphase cells using MBS602417. The spindle, centrosome and centromeric regions are stained. DNA is counterstained with propidium iodide (red).)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of HeLa cells were treated with colcemid (green), or untreated (grey) using MBS602417.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of HeLa cells were treated with colcemid (green), or untreated (grey) using MBS602417.)
Testing Data
Testing Data
Related Product Information for anti-MPM2, Ser/Thr, Pro antibody
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho aa-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells. This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Product Categories/Family for anti-MPM2, Ser/Thr, Pro antibody
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Product Notes
The MPM2, Ser/Thr, Pro (Catalog #AAA602417) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MPM2, Ser/Thr, Pro, phosphorylated (Mitotic Protein Monoclonal#2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MPM2, Ser/Thr, Pro can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunocytochemistry, ELISA, Immunohistochemistry, Immunoprecipitation and Flow Cytometry. Dilution: Western Blot: 0.5-2ug/ml detects mitosis-associated proteins in colcemid treated (0.05ug/ml) human HeLa carcinoma cells. Colcemid-treated HeLa cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with M4685. Proteins were visualized using an IgG (HRP) goat anti-mouse antibody and ECL. Immunocytochemistry: 5-15ug/ml shows positive staining of mitotic cells. ELISA: Used in a non-radioactive, dissociation-enhanced time resolved fluoroimmunoassay (DELPHIA). Active enzymes were used to phosphorylate substrate peptides. The phosphorylated peptides were detected using MPM2 in conjunction with an europium (Eu3+) labeled secondary antibody. The phosphorylated peptides detected include: Serine/Threonine Kinase Substrate Peptide, Recombinant, Human (Biotin). Immunoprecipitation: Reported by an independent laboratory. Researchers should empirically determine the suitability of the MPM2, Ser/Thr, Pro for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MPM2, Ser/Thr, Pro, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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