Mouse F(ab')2 Anti-Human CD19 Secondary Antibody
Mouse F(ab')2 Anti-Human CD19-FITC
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Synonyms
Mouse F(ab')2 Anti-Human CD19; Monoclonal Secondary Antibody; Mouse F(ab')2 Anti-Human CD19-FITC; Mouse F(ab')2 Anti-Human CD19 - FITC; Mouse F(ab')2 Anti-Human CD19 secondary antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
SJ25-C1
Specificity
CD19
CD19 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is expressed at all stages of B cell differentiation except terminally differentiated plasma cells. It is also present on the cell surface of follicular dendritic cells. CD19 associates with CD21, CD81, Leu 13 and/or MHC Class II molecules to form a signal transduction complex on the B cell surface. This signaling complex modulates the activation threshold for the B cell antigen receptor (BCR).1-5
CD19 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is expressed at all stages of B cell differentiation except terminally differentiated plasma cells. It is also present on the cell surface of follicular dendritic cells. CD19 associates with CD21, CD81, Leu 13 and/or MHC Class II molecules to form a signal transduction complex on the B cell surface. This signaling complex modulates the activation threshold for the B cell antigen receptor (BCR).1-5
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for Mouse F(ab')2 Anti-Human CD19 secondary antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections)
Application Notes
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
References
1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD19 Section, Academic Press, New York, p. 179.
3. Tedder, T.F., L.J. Zhou, and P. Engel. 1994. Immunol. Today 14:437.
4. Fearon, D.T., and R.H. Carter. 1995. Annu. Rev. Immunol. 13:127.
5. Doody, G.M., P.W. Dempsey, and D.T. Fearson. 1996. Curr. Opin. Immunol. 8:378.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD19 Section, Academic Press, New York, p. 179.
3. Tedder, T.F., L.J. Zhou, and P. Engel. 1994. Immunol. Today 14:437.
4. Fearon, D.T., and R.H. Carter. 1995. Annu. Rev. Immunol. 13:127.
5. Doody, G.M., P.W. Dempsey, and D.T. Fearson. 1996. Curr. Opin. Immunol. 8:378.
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Product Notes
The Mouse F(ab')2 Anti-Human CD19 (Catalog #AAA674122) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse F(ab')2 Anti-Human CD19-FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mouse F(ab')2 Anti-Human CD19 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Frozen Sections). Flow Cytometry Suggested Dilution: 10 uL/10^6 cells. Researchers should empirically determine the suitability of the Mouse F(ab')2 Anti-Human CD19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse F(ab')2 Anti-Human CD19, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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