Rabbit MMP12 Monoclonal Antibody | anti-MMP12 antibody

MMP12 Rabbit Monoclonal Antibody

Cat. Num. AAA3014092

• Gene Names: MMP12; ME; HME; MME; MMP-12
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: MMP12; Monoclonal Antibody; MMP12 Rabbit Monoclonal Antibody; Macrophage metalloelastase; MME; 3.4.24.65; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; HME; anti-MMP12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of MMP12 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 470

• Applicable Applications for anti-MMP12 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:2000; ICC: 1:50-1:200; IF: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MMP12 Rabbit mAb at 1:1000 dilutionLane1:A549 whole cell lysateLane2:Hela whole cell lysateLane3:MCF7 whole cell lysateLane4:THP1 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinomna tissue using anti-MMP12 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. Counter stained with hematoxylin.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of A549 cells with MMP12 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-MMP12 antibody

Matrix metallopeptidase 12 (MMP-12) is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3); it contains a propeptide with autoinhibitory cysteine switch site, a well conserved zinc site, hinge region and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, and this breaks down to cascade of active forms, ending with the common 22 kDa form.Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced.

NCBI and Uniprot Product Information

• NCBI GI #: 73858572
• NCBI GeneID: 4321
• NCBI Accession #: NP_002417.2
• NCBI GenBank Nucleotide #: NP_002417.2
• UniProt Accession #: P39900
• Molecular Weight: ~ 40, 54kDa
• NCBI Official Full Name: macrophage metalloelastase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 12
• NCBI Official Symbol: MMP12
• NCBI Official Synonym Symbols: ME; HME; MME; MMP-12
• NCBI Protein Information: macrophage metalloelastase
• UniProt Protein Name: Macrophage metalloelastase
• Protein Family: Macrophage metalloelastase
• UniProt Gene Name: MMP12
• UniProt Synonym Gene Names: HME; MME; ME; hME; MMP-12
• UniProt Entry Name: MMP12_HUMAN

NCBI Description

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

Uniprot Description

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.24.65; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells