Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit | MMP-12 elisa kit

Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit

Cat. Num. AAA9715263

• Gene Names: MMP12; ME; HME; MME; MMP-12
• Reactivity: Human
• Synonyms: Matrix Metalloproteinase 12 (MMP-12); Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit; MMP12; HME; MGC138506; MME; macrophage elastase; macrophage metalloelastase; matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); MMP-12 elisa kit

• Reactivity: Human
• Sequence Length: 470

• Assay Type: Sandwich
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for MMP-12 elisa kit

Principle of the Assay: Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP-12 in samples. An antibody specific for MMP-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-12 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MMP-12 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MMP-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background/Introduction: MMP-12 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. MMP-12 may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). MMP-12 is part of a cluster of MMP genes on chromosome 11.

NCBI and Uniprot Product Information

• NCBI GI #: 73858572
• NCBI GeneID: 4321
• NCBI Accession #: NP_002417.2
• NCBI GenBank Nucleotide #: NP_002417.2
• UniProt Accession #: P39900
• NCBI Official Full Name: macrophage metalloelastase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 12
• NCBI Official Symbol: MMP12
• NCBI Official Synonym Symbols: ME; HME; MME; MMP-12
• NCBI Protein Information: macrophage metalloelastase
• UniProt Protein Name: Macrophage metalloelastase
• UniProt Gene Name: MMP12
• UniProt Synonym Gene Names: HME; MME; ME; hME; MMP-12
• UniProt Entry Name: MMP12_HUMAN

NCBI Description

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

Uniprot Description

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.24.65; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells