Mouse anti-Human MLKL Monoclonal Antibody | anti-MLKL antibody

Phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8)

Cat. Num. AAA9700548

• Gene Names: MLKL; hMLKL
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: MLKL; Monoclonal Antibody; Phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8); Mouse Anti-phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8); anti-MLKL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: phospho-MLKL (S358) protein detects endogenous levels of MLKL.
• Purity/Purification: Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 263

• Applicable Applications for anti-MLKL antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:200
• Immunogen: Synthetic Peptide of phospho-MLKL (S358)
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Fig.1. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue. 1, phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

Related Product Information for anti-MLKL antibody

MLKL (mixed lineage kinase domain like) belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of MLKL inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for MLKL.

NCBI and Uniprot Product Information

• NCBI GI #: 215820622
• NCBI GeneID: 197259
• NCBI Accession #: NP_001135969.1
• NCBI GenBank Nucleotide #: NP_001135969.1
• UniProt Accession #: Q8NB16
• NCBI Official Full Name: mixed lineage kinase domain-like protein isoform 2
• NCBI Official Synonym Full Names: mixed lineage kinase domain like pseudokinase
• NCBI Official Symbol: MLKL
• NCBI Official Synonym Symbols: hMLKL
• NCBI Protein Information: mixed lineage kinase domain-like protein
• UniProt Protein Name: Mixed lineage kinase domain-like protein
• Protein Family: Mixed lineage kinase domain-like protein
• UniProt Gene Name: MLKL
• UniProt Entry Name: MLKL_HUMAN

NCBI Description

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; TKL-Unique family

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein binding; protein complex binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: protein amino acid phosphorylation