Western Blot (WB)
(Anti- MLH1 Picoband antibody, MBS177968, Western blottingAll lanes: Anti MLH1 (MBS177968) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 84KDObserved bind size: 84KD)
MLH1 Polyclonal Antibody | anti-MLH1 antibody
Anti-MLH1 Antibody
Western Blot (WB)
(Anti- MLH1 Picoband antibody, MBS177968, Western blottingAll lanes: Anti MLH1 (MBS177968) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 84KDObserved bind size: 84KD)
Western Blot (WB)
(Anti- MLH1 Picoband antibody, MBS177968, Western blottingAll lanes: Anti MLH1 (MBS177968) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 84KDObserved bind size: 84KD)
Background: MutL homolog 1, colon cancer, nonpolyposis type 2 (E Coli) is a protein that in humans is encoded by the MLH1 gene located on Chromosome 3. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E Coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
NCBI and Uniprot Product Information
NCBI Description
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009]
Uniprot Description
MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer.
Protein type: DNA repair, damage; Tumor suppressor
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: chiasma; male germ cell nucleus; membrane; MutLalpha complex; nucleoplasm; nucleus; synaptonemal complex
Molecular Function: ATP binding; ATPase activity; chromatin binding; guanine/thymine mispair binding; MutSalpha complex binding; protein binding; single-stranded DNA binding
Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; double-strand break repair via nonhomologous end joining; female meiosis chromosome segregation; isotype switching; male meiosis chromosome segregation; meiotic metaphase I plate congression; mismatch repair; negative regulation of mitotic recombination; oogenesis; poly(A) tail shortening; resolution of meiotic joint molecules as recombinants; somatic hypermutation of immunoglobulin genes; spermatogenesis; spindle midzone assembly involved in meiosis; synapsis; telomere clustering
Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 2; Mismatch Repair Cancer Syndrome; Muir-torre Syndrome
Research Articles on MLH1
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Product Notes
The MLH1 mlh1 (Catalog #AAA177968) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-MLH1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MLH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the MLH1 mlh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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