Mouse anti-Human MKP3 / DUSP6 Monoclonal Antibody | anti-MKP3 antibody

Mouse Monoclonal [clone 3G2] (IgG1,k) to Human MKP3 / DUSP6

Cat. Num. AAA246518

• Gene Names: DUSP6; HH19; MKP3; PYST1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: MKP3 / DUSP6; Monoclonal Antibody; Mouse Monoclonal [clone 3G2] (IgG1; k) to Human MKP3 / DUSP6; Anti-MKP3 / DUSP6 Antibody (clone 3G2) IHC-plus; DUSP6; Dual specificity phosphatase 6; MAP kinase phosphatase 3; MKP3; PYST1; RVH6; Mapk phosphatase 3; MKP-3; Phosphatase VH6; Human MKP3; anti-MKP3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Sequence Length: 381

• Applicable Applications for anti-MKP3 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA), RNA interference
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), RNAi, WB; Usage: Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).
• Target Species: Human
• Immunogen Description: DUSP6 (AAH03143, 1 a.a. ~ 382 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: MKP3 / DUSP6 antibody was raised against dUSP6 (AAH03143, 1 a.a. ~ 382 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-DUSP6 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-DUSP6 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to DUSP6 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(Western blot of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody, clone 3G2.)

RNA interference

(Western blot of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody clone 3G2. GAPDH (36.1 kD) used as specificity a.)

ELISA (EIA)

(Detection limit for recombinant GST tagged DUSP6 is approximately 1 ng/ml as a capture antibody.)

Related Product Information for anti-MKP3 antibody

DUSP6 is a dual specificity MAP kinase phosphatase which has been shown to block the phosphorylation and enzymatic activation by mitogens of ERKs, especially ERK2. It has been shown to affect Schwann cell differentiation, apoptosis of olfactory neuroepithelium, synaptic remodeling, and neuronal differentiation and death. At least two mRNA splice variants have been reported.

Product Categories/Family for anti-MKP3 antibody

Family: Protein Phosphatase; Subfamily: Dual specificity MKP

NCBI and Uniprot Product Information

• NCBI GI #: 42764683
• NCBI GeneID: 1848
• NCBI Accession #: NP_001937.2
• NCBI GenBank Nucleotide #: NM_001946.3
• UniProt Accession #: Q16828; O75109; Q53Y75; Q9BSH6
• Molecular Weight: 26,468 Da
• NCBI Official Full Name: dual specificity protein phosphatase 6 isoform a
• NCBI Official Synonym Full Names: dual specificity phosphatase 6
• NCBI Official Symbol: DUSP6
• NCBI Official Synonym Symbols: HH19; MKP3; PYST1
• NCBI Protein Information: dual specificity protein phosphatase 6; MAP kinase phosphatase 3; dual specificity protein phosphatase PYST1; serine/threonine specific protein phosphatase; mitogen-activated protein kinase phosphatase 3
• UniProt Protein Name: Dual specificity protein phosphatase 6
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP6
• UniProt Synonym Gene Names: MKP3; PYST1; MAP kinase phosphatase 3; MKP-3
• UniProt Entry Name: DUS6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

MKP-3: a non-receptor, dual-specificity phosphoprotein phosphatase. Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This protein inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, dual-specificity; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: response to drug; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of fibroblast growth factor receptor signaling pathway; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to nitrosative stress; dorsal/ventral pattern formation; toll-like receptor signaling pathway; innate immune response; regulation of endodermal cell fate specification; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway; inactivation of MAPK activity

Disease: Hypogonadotropic Hypogonadism 19 With Or Without Anosmia