Rabbit anti-Human, Mouse DDX47 Polyclonal Antibody | anti-DDX47 antibody

DDX47, CT (DDX47, Probable ATP-dependent RNA helicase DDX47, DEAD box protein 47) (Biotin)

Cat. Num. AAA6291488

• Gene Names: DDX47; RRP3; E4-DBP; HQ0256; MSTP162
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX47; Polyclonal Antibody; CT (DDX47; Probable ATP-dependent RNA helicase DDX47; DEAD box protein 47) (Biotin); DEAD box protein 47; anti-DDX47 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DDX47 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DDX47 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-388 amino acids from the C-terminal region of human DDX47.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DDX47 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DDX47 antibody detected the DDX47 protein (arrow).)

Flow Cytometry (FC/FACS)

(DDX47 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(DDX47 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the DDX47 antibody detected the DDX47 protein (arrow).)

Product Categories/Family for anti-DDX47 antibody

Antibodies; DEAD Box Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 41327774
• NCBI GeneID: 51202
• NCBI Accession #: NP_057439.2; NP_957518.1
• NCBI GenBank Nucleotide #: NM_016355
• UniProt Accession #: Q9H0S4; Q96GM0; Q96NV8; Q9UI98; B3KXP4; G5E955
• Molecular Weight: 45,169 Da
• NCBI Official Full Name: Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 (DDX47), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
• NCBI Official Symbol: DDX47
• NCBI Official Synonym Symbols: RRP3; E4-DBP; HQ0256; MSTP162
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX47; DEAD box polypeptide 47; DEAD box protein 47; E4-DEAD box protein
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX47
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX47
• UniProt Entry Name: DDX47_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]