Rabbit MKLP1 Monoclonal Antibody | anti-MKLP1 antibody

MKLP1 Rabbit Monoclonal Antibody

Cat. Num. AAA3014128

• Gene Names: KIF23; CHO1; KNSL5; MKLP1; MKLP-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A affinity purified
• Synonyms: MKLP1; Monoclonal Antibody; MKLP1 Rabbit Monoclonal Antibody; anti-MKLP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of MKLP1 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 856

• Applicable Applications for anti-MKLP1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000-1:5000; ICC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MKLP1 Rabbit mAb at 1:1000 dilutionLane1:A549 whole cell lysateLane2:Hela whole cell lysate)

Immunocytochemistry (ICC)

(ICC staining MKLP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunocytochemistry (ICC)

(ICC staining MKLP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Related Product Information for anti-MKLP1 antibody

MKLP-1 (mitotic kinesin-like protein-1), represents a novel kinesin. Sequence analysis has also been shown to predict that MKLP-1 possesses features typical of nuclear proteins. Immunocytological studies have demonstrated that MKLP-1 moves from the nucleus early in mitosis and then to the midbody after cytokinesis. MKLP-1 has been shown to bundle antiparallel microtubules in vitro and to move them at rates comparable to spindle elongation in vivo. A hamster homolog of MKLP-1, designated CHO1 antigen, has also been isolated. Although apparently functionally equivalent with respect to microtubule bundling activity, there are significant differences between the human and hamster proteins at their C-termini, possibly due to alternative splicing or the presence of more than one MKLP-1 locus.

NCBI and Uniprot Product Information

• NCBI GI #: 6754472
• NCBI GeneID: 9493
• NCBI Accession #: NP_004847.2
• NCBI GenBank Nucleotide #: NP_004847.2
• UniProt Accession #: Q02241
• NCBI Official Full Name: kinesin-like protein KIF23 isoform 2
• NCBI Official Synonym Full Names: kinesin family member 23
• NCBI Official Symbol: KIF23
• NCBI Official Synonym Symbols: CHO1; KNSL5; MKLP1; MKLP-1
• NCBI Protein Information: kinesin-like protein KIF23
• UniProt Protein Name: Kinesin-like protein KIF23
• UniProt Gene Name: KIF23
• UniProt Synonym Gene Names: KNSL5; MKLP1

NCBI Description

The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.