EN1/Engrailed1 Recombinant Protein | EN1 recombinant protein

Recombinant Human EN1/Engrailed1 protein, N- His tag

Cat. Num. AAA157487

• Purity: >90% as determined by SDS-PAGE
• Synonyms: EN1/Engrailed1; Recombinant Human EN1/Engrailed1 protein; N- His tag; Homeobox protein en-1; Hu-En-1; EN1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from 0.9%NaCl, PSA 1.5mg/ml, 0.02% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Source: Human
• Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
• Construction: A DNA sequence encoding the human EN1 (Met1-Glu392) was fused with the C-terminal His Tag
• Preparation and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for ~12 months from the date of receipt.; Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise

SDS-Page

Product Categories/Family for EN1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 126090909
• NCBI GeneID: 2019
• NCBI Accession #: NP_001417.3
• NCBI GenBank Nucleotide #: NM_001426.3
• UniProt Accession #: Q05925; Q4ZG44
• Molecular Weight: 40,115 Da
• NCBI Official Full Name: homeobox protein engrailed-1
• NCBI Official Synonym Full Names: engrailed homeobox 1
• NCBI Official Symbol: EN1
• NCBI Protein Information: homeobox protein engrailed-1; hu-En-1; engrailed homolog 1; homeobox protein en-1
• UniProt Protein Name: Homeobox protein engrailed-1
• Protein Family: Homeobox protein
• UniProt Gene Name: EN1
• UniProt Synonym Gene Names: Homeobox protein en-1; Hu-En-1
• UniProt Entry Name: HME1_HUMAN

NCBI Description

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

Uniprot Description

EN1: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

Protein type: Transcription factor; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: membrane; nucleus

Biological Process: embryonic forelimb morphogenesis; anatomical structure morphogenesis; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; dorsal/ventral pattern formation; pigmentation; midbrain-hindbrain boundary development; midbrain development; neuron development; positive regulation of transcription from RNA polymerase II promoter; skeletal development; proximal/distal pattern formation; hindbrain development