Mammalian EZCellTM Cell Cycle Analysis Kit Assay Kit | CBL assay kit

EZCellTM Cell Cycle Analysis Kit

Cat. Num. AAA841661

• Gene Names: CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
• Reactivity: Mammalian
• Synonyms: EZCellTM Cell Cycle Analysis Kit; CBL assay kit

• Reactivity: Mammalian
• Sequence Length: 906

• Application Notes: Analysis of cell cycle regulation in response to growth factors, cytokines, mitogens, and nutrients, etc. ; Monitoring of cell cycle progression; Study effects of drugs which affect cell growth and division
• Samples: Adherent or Suspension cells
• Detection Method: Flow cytometry (Ex 488 nm)
• Features & Benefits: Highly sensitive flow cytometry-based method to detect and monitor cells at various stages of the cell cycle; Simple & High throughput-adaptable ; Easy tool for screening compounds that affect cell growth and division
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CBL assay kit

Product Information: Highly Sensitive, HTS compatible, Flow-cytometry assay to identify cells at different stages of the Cell Cycle. 100 Assays

Background: Cell cycle is a ubiquitous, complex sequence of events leading to growth and proliferation of cells. Cell Cycle progression is tightly regulated due to its involvement in development, DNA damage and repair, etc. Anomalies in cell cycle progression can lead to tissue hyperplasia and diseases such as cancer. Cell cycle can be subdivided into interphase (G0/G1, S and G2) and mitotic (M) phase (prophase, metaphase, anaphase and telophase). MyBioSource's Cell Cycle Analysis Kit provides a quick and easy method to detect the number of cells in a cell population, which are at a specific stage of the cell cycle. Our kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry. Our method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.

Summary: Detection method - Flow cytometry (Ex 488 nm)
Species reactivity- Mammalian
Applications-
Analysis of cell cycle regulation in response to growth factors, cytokines, mitogens, and nutrients, etc.
Monitoring of cell cycle progression
Study effects of drugs which affect cell growth and division

Product Categories/Family for CBL assay kit

Home; Cell-based Assays; Cell Cycle Assay Kit

NCBI and Uniprot Product Information

• NCBI GI #: 251757253
• NCBI GeneID: 867
• Molecular Weight: 99,633 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL
• NCBI Official Synonym Full Names: Cbl proto-oncogene, E3 ubiquitin protein ligase
• NCBI Official Symbol: CBL
• NCBI Official Synonym Symbols: CBL2; NSLL; C-CBL; RNF55; FRA11B
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: CBL
• UniProt Synonym Gene Names: CBL2; RNF55
• UniProt Entry Name: CBL_HUMAN

NCBI Description

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012]

Uniprot Description

CBL: an adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Participates in signal transduction in hematopoietic cells.

Protein type: Oncoprotein; EC 6.3.2.-; Adaptor/scaffold; Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.19; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; plasma membrane; flotillin complex; nucleus; cytosol

Molecular Function: signal transducer activity; protein binding; ephrin receptor binding; phosphotyrosine binding; zinc ion binding; ubiquitin-protein ligase activity; calcium ion binding; transcription factor activity; SH3 domain binding; ligase activity

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; cell surface receptor linked signal transduction; fibroblast growth factor receptor signaling pathway; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; positive regulation of receptor-mediated endocytosis; negative regulation of apoptosis

Disease: Noonan Syndrome-like Disorder With Or Without Juvenile Myelomonocytic Leukemia