Chicken VEGF-C ELISA Kit | VEGF-C elisa kit

Chicken VEGF-C (Vascular Endothelial Growth Factor C) ELISA Kit

Cat. Num. AAA2502435

• Gene Names: VEGFC; VRP; Flt4-L; LMPH1D
• Reactivity: Chicken
• Synonyms: VEGF-C; Chicken VEGF-C (Vascular Endothelial Growth Factor C) ELISA Kit; VEGF-C elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken VEGF-C. No significant cross-reactivity or interference between Chicken VEGF-C and analogues was observed.
• Sequence Length: 419

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGF-C elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken VEGF-C concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VEGF-C. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VEGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VEGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VEGF-C. You can calculate the concentration of VEGF-C in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.4
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 46,883 Da
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L; LMPH1D
• NCBI Protein Information: vascular endothelial growth factor C; FLT4 ligand DHM; vascular endothelial growth factor-related protein
• UniProt Protein Name: Vascular endothelial growth factor C
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]

Uniprot Description

VEGFC: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Homodimer; non-covalent and antiparallel. Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. Belongs to the PDGF/VEGF growth factor family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted; Cell cycle regulation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein binding; growth factor activity; vascular endothelial growth factor receptor 3 binding; chemoattractant activity

Biological Process: response to drug; substrate-bound cell migration; platelet activation; positive regulation of neuroblast proliferation; positive regulation of blood vessel endothelial cell migration; signal transduction; positive regulation of protein amino acid autophosphorylation; induction of positive chemotaxis; negative regulation of cell proliferation; organ morphogenesis; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; morphogenesis of embryonic epithelium; platelet degranulation; positive regulation of protein secretion; positive chemotaxis; positive regulation of cell division; regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of cell proliferation; angiogenesis; negative regulation of blood pressure; positive regulation of cell-matrix adhesion; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of epithelial cell proliferation

Disease: Lymphedema, Hereditary, Id