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Mouse Mitochondrial Ribosomal Protein S25 Monoclonal Antibody | anti-MRPS25 antibody

Mouse Anti Human Mitochondrial Ribosomal Protein S25

Gene Names
MRPS25; RPMS25; MRP-S25
Applications
ELISA, Western Blot
Purity
MRPS25 antibody was purified by protein-A affinity chromatography.
Synonyms
Mitochondrial Ribosomal Protein S25; Monoclonal Antibody; Mouse Anti Human Mitochondrial Ribosomal Protein S25; MRPS25 Antibody; Mouse Anti Human; Mitochondrial 28S Ribosomal Protein S25; 28S Ribosomal Protein S25 Mitochondrial; MRP-S25;; anti-MRPS25 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a heavy chain and Kappa light chain
Clone Number
PAT38E7A
Purity/Purification
MRPS25 antibody was purified by protein-A affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Sterile filtered colorless solution.
Sequence Length
173
Applicable Applications for anti-MRPS25 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.
Immunogen
Anti-human MRPS25 mAb, clone PAT38E7A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MRPS25 protein 1-173 amino acids purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-MRPS25 antibody
Introduction: MRPS25, a 28S subunit protein, is a member of the ribosomal protein S5P family. An equivalent pseudogene is located on chromosome 4.
Product Categories/Family for anti-MRPS25 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
14,953 Da
NCBI Official Full Name
Mitochondrial ribosomal protein S25
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S25
NCBI Official Symbol
MRPS25
NCBI Official Synonym Symbols
RPMS25; MRP-S25
NCBI Protein Information
28S ribosomal protein S25, mitochondrial; S25mt; mitochondrial 28S ribosomal protein S25
UniProt Protein Name
28S ribosomal protein S25, mitochondrial
Protein Family
UniProt Gene Name
MRPS25
UniProt Synonym Gene Names
RPMS25; MRP-S25; S25mt
UniProt Entry Name
RT25_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS25: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: mitochondrion; mitochondrial inner membrane; ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis

Research Articles on MRPS25

Similar Products

Product Notes

The MRPS25 mrps25 (Catalog #AAA140416) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mitochondrial Ribosomal Protein S25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the MRPS25 mrps25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitochondrial Ribosomal Protein S25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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