Western Blot (WB)
(The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRPS25 antibody (1:500 ~ 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human MRPS25 Monoclonal Antibody | anti-MRPS25 antibody
MRPS25 antibody
Gene Names
MRPS25; RPMS25; MRP-S25
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
MRPS25; Monoclonal Antibody; MRPS25 antibody; 28S ribosomal protein S25 mitochondrial; 28S ribosomal protein S25; mitochondrial; mitochondrial ribosomal protein S25; MRP-S25; RPMS25; anti-MRPS25 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT38E7
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
173
Applicable Applications for anti-MRPS25 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500 ~ 1:5000.
Recommended starting dilution is 1:1000.
Recommended starting dilution is 1:1000.
Antigen Species
Human
Immunogen
Recombinant human MRPS25 (1-173aa) purified from E. coli
Clone Note
Anti-human MRPS25 mAb, clone AT38E7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MRPS25 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRPS25 antibody (1:500 ~ 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRPS25 antibody (1:500 ~ 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-MRPS25 antibody
MRPS25 is a 28S subunit protein that belongs to the ribosomal protein S5P family. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. A pseudogene corresponding to this gene is found on chromosome 4
Product Categories/Family for anti-MRPS25 antibody
References
Koc EC, Burkhart W, Blackburn K, et al. (2000). J. Biol. Chem. 275 (42): 32585-91.; Zhang Z, Gerstein M (2003). Genomics 81 (5): 468-80.; Cavdar Koc E, et al. (May 2001). J Biol Chem 276 (22): 19363-74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,953 Da
NCBI Official Full Name
28S ribosomal protein S25, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S25
NCBI Official Symbol
MRPS25
NCBI Official Synonym Symbols
RPMS25; MRP-S25
NCBI Protein Information
28S ribosomal protein S25, mitochondrial
UniProt Protein Name
28S ribosomal protein S25, mitochondrial
Protein Family
UniProt Gene Name
MRPS25
UniProt Synonym Gene Names
RPMS25; MRP-S25; S25mt
NCBI Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Research Articles on MRPS25
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Product Notes
The MRPS25 mrps25 (Catalog #AAA200271) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MRPS25 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRPS25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500 ~ 1:5000. Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the MRPS25 mrps25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPS25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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