VEGFR2 / Flk-1 / CD309 / KDR recombinant protein

Recombinant Rat VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc tag)

Cat. Num. AAA2547252

• Gene Names: Kdr; Vegfr-2
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: VEGFR2 / Flk-1 / CD309 / KDR; Recombinant Rat VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc tag); KDR; VEGFR2 / Flk-1 / CD309 / KDR recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1343

• Application Notes: The recombinant rat KDR comprises 982 amino acids and has a predicted molecular mass of 110 kDa.
• Predicted N Terminal: Ala 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for VEGFR2 / Flk-1 / CD309 / KDR recombinant protein

Background: VEGFR2, also called as KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 was shown to be the primary signal transducer for angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. It has been shown that VEGFR2 is expressed mainly in the endothelial cells, and the expression is upregulated in the tumor vasculature. Thus the inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of diseases involving angiogenesis. VEGFR2 transduces the major signals for angiogenesis via its strong tyrosine kinase activity. However, unlike other representative tyrosine kinase receptors, VEGFR2 does not use the Ras pathway as a major downstream signaling but rather uses the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP)-kinase activation and DNA synthesis. VEGFR2 is a direct and major signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy, in cooperation with many other signaling partners; thus, VEGFR2 and its downstream signaling appear to be critical targets for the suppression of these diseases. VEGF and VEGFR2-mediated survival signaling is critical to endothelial cell survival, maintenance of the vasculature and alveolar structure and regeneration of lung tissue. Reduced VEGF and VEGFR2 expression in emphysematous lungs has been linked to increased endothelial cell death and vascular regression.

Description: A DNA sequence encoding the rat KDR (AAH87029.1) (Met1-Glu760) was expressed with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 56269800
• NCBI GeneID: 25589
• Molecular Weight: 150,394 Da
• NCBI Official Full Name: Kdr protein
• NCBI Official Synonym Full Names: kinase insert domain receptor
• NCBI Official Symbol: Kdr
• NCBI Official Synonym Symbols: Vegfr-2
• NCBI Protein Information: vascular endothelial growth factor receptor 2
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• UniProt Gene Name: Kdr
• UniProt Synonym Gene Names: Flk1; VEGFR-2; FLK-1

NCBI Description

receptor for vascular endothelial growth factor (VEGF) [RGD, Feb 2006]

Uniprot Description

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; VEGFR family

Chromosomal Location of Human Ortholog: 14p11

Cellular Component: cell junction; cell soma; cell surface; cytoplasm; early endosome; endoplasmic reticulum; endosome; external side of plasma membrane; Golgi apparatus; integral to plasma membrane; lipid raft; neuron projection; nucleus; plasma membrane

Molecular Function: growth factor binding; integrin binding; protein binding; protein-tyrosine kinase activity; vascular endothelial growth factor receptor activity

Biological Process: aging; alveolus development; branching morphogenesis of a tube; calcium ion homeostasis; cell fate commitment; cell maturation; cell migration; cell migration during sprouting angiogenesis; elevation of cytosolic calcium ion concentration; embryonic hemopoiesis; endothelial cell differentiation; hemopoiesis; hyaluronan metabolic process; lung development; lymph vessel development; male gonad development; negative regulation of apoptosis; negative regulation of neuron apoptosis; neurite morphogenesis; ovarian follicle development; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of BMP signaling pathway; positive regulation of calcium-mediated signaling; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of focal adhesion formation; positive regulation of long-term neuronal synaptic plasticity; positive regulation of macroautophagy; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of mitochondrial depolarization; positive regulation of neurogenesis; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of positive chemotaxis; positive regulation of protein amino acid phosphorylation; positive regulation of TOR signaling pathway; positive regulation of vasodilation; post-embryonic camera-type eye morphogenesis; protein amino acid autophosphorylation; regulation of cell shape; regulation of endothelial cell differentiation; response to drug; response to hypoxia; surfactant homeostasis; vascular endothelial growth factor receptor signaling pathway; vasculogenesis