Mouse anti-Human MIP-3 alpha Monoclonal Antibody | anti-MIP-3 alpha antibody
Mouse Anti-Human MIP-3 alpha
In a sandwich ELISA (assuming 100 ul/well), a concentration of 2 - 4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human MIP-3 alpha when used with biotinylated antigen affinity purified anti-human MIP-3 alpha as the detection antibody at a concentration of approximately 1 -2 ug/ml.
Western Blot:
To detect Human MIP-3 alpha by Western Blot analysis this antibody can be used at a concentration of 0.2 - 0.4 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3 alpha is 0.25 - 0.5 ng/lane, under non-reducing conditions.
Optimal dilutions should be determined by each laboratory for each application.
The reconstituted antibody is stable for at least 2 weeks at 2-8°C.
Frozen aliquots are stable for at least 6 months when stored at -20°C.
Avoid repeated freeze and thaw cycles.
NCBI and Uniprot Product Information
NCBI Description
This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Ref.8
Subunit structure: Binds to and activate CCR6.
Subcellular location: Secreted.
Tissue specificity: Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
Induction: By bacterial lipopolysaccharides (LPS), TNF and IFNG/IFN-gamma. Induced by phorbol myristate acetate (PMA) in U-937 cell line and bowes melanoma. Repressed by IL10/interleukin-10.
Post-translational modification: C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
Research Articles on MIP-3 alpha
Similar Products
Product Notes
The MIP-3 alpha ccl20 (Catalog #AAA690971) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human MIP-3 alpha reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIP-3 alpha can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot (WB). ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2 - 4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human MIP-3 alpha when used with biotinylated antigen affinity purified anti-human MIP-3 alpha as the detection antibody at a concentration of approximately 1 -2 ug/ml. Western Blot: To detect Human MIP-3 alpha by Western Blot analysis this antibody can be used at a concentration of 0.2 - 0.4 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3 alpha is 0.25 - 0.5 ng/lane, under non-reducing conditions. Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the MIP-3 alpha ccl20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIP-3 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
