Rabbit WDR48 Polyclonal Antibody | anti-WDR48 antibody

WDR48 Polyclonal Antibody

Cat. Num. AAA3008725

• Gene Names: WDR48; P80; UAF1; SPG60
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: WDR48; Polyclonal Antibody; WDR48 Polyclonal Antibody; P80; UAF1; SPG60; anti-WDR48 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: WDR48 polyclonal antibody detects endogenous levels of WDR48 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 677

• Applicable Applications for anti-WDR48 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human WDR48.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using WDR48 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat liver using WDR48 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using WDR48 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-WDR48 antibody

WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids, which commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms involving signal transduction, apoptosis, transcriptional regulation and cell cycle control. WD repeats serve as sites for protein-protein interaction and some seem to mediate the assembly of protein complexes. With eight WD repeats, WDR48 (WD repeat-containing protein 48), also known as USP1-associated factor 1 and p80, is a 677 amino acid protein that functions to regulate deubiquitinating complexes via activation of USP1, USP12 and USP46. WDR48 enhances deubiquitination by increasing catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. WDR48 is ubiquitously expressed and is mainly localized to the cytoplasm. There are five isoforms of WDR48 that are expressed as a result of alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 74760390
• NCBI GeneID: 57599
• UniProt Accession #: Q8TAF3
• Molecular Weight: ~ 76kDa
• NCBI Official Full Name: WD repeat-containing protein 48
• NCBI Official Synonym Full Names: WD repeat domain 48
• NCBI Official Symbol: WDR48
• NCBI Official Synonym Symbols: P80; UAF1; SPG60
• NCBI Protein Information: WD repeat-containing protein 48
• UniProt Protein Name: WD repeat-containing protein 48
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: WDR48
• UniProt Synonym Gene Names: KIAA1449; UAF1
• UniProt Entry Name: WDR48_HUMAN

NCBI Description

The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016]

Uniprot Description

WDR48: an ubiquitous lysosomal WD40 repeat protein. May play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP.

Protein type: Ubiquitin conjugating system; Vesicle

Chromosomal Location of Human Ortholog: 3p21.33

Cellular Component: intracellular membrane-bound organelle; lysosome; nucleus

Molecular Function: protein binding

Biological Process: skin development; protein deubiquitination; viral reproduction; skeletal morphogenesis; single fertilization; multicellular organism growth; homeostasis of number of cells; embryonic organ development; spermatogenesis; double-strand break repair via homologous recombination; positive regulation of epithelial cell proliferation