Rabbit anti-Human MICB Monoclonal Antibody | anti-MICB antibody

Recombinant Anti-MICB Antibody, Rabbit Monoclonal

Cat. Num. AAA8103814

• Gene Names: MICB; PERB11.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A
• Synonyms: MICB; Monoclonal Antibody; Recombinant Anti-MICB Antibody; Rabbit Monoclonal; MICB Antibody; Rabbit MAb; MHC class I polypeptide-related sequence B; Anti-PERB11.2 Antibody; anti-MICB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 102
• Specificity: Human MICB
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MICB antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human MICB protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human MICB (rh MICB).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MICB rabbit monoclonal antibody at 1:500 dilutionLane A: U87MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:40 kDaObserved band size:67 kDa)

Related Product Information for anti-MICB antibody

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+alphabeta T cells, and gammadelta T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

References

Bauer, S. et al., 1999, Science. 285:727-729. Braud, V.M. et al., 1999, Curr. Opin. Immunol. 11: 100-108. Groh, V. et al., 2001, Nature Immunol. 2: 255-260. Stephens, H., 2001, Trends Immunol. 22: 378-385. Borrego, F. et al., 2002, Mol. Immunol. 38: 637-660. Groh, V. et al., 2002, Nature. 419:734-738.

NCBI and Uniprot Product Information

• NCBI GI #: 167857788
• NCBI GeneID: 4277
• NCBI Accession #: NP_005922.2
• NCBI GenBank Nucleotide #: NM_005931.4
• UniProt Accession #: Q29980; O14499; O14500; O19798; O19799; O19800; O19801; O19802; O19803; O78099; A6NP85; B0UZ10
• Molecular Weight: 42,646 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence B isoform 1
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence B
• NCBI Official Symbol: MICB
• NCBI Official Synonym Symbols: PERB11.2
• NCBI Protein Information: MHC class I polypeptide-related sequence B; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• UniProt Protein Name: MHC class I polypeptide-related sequence B
• Protein Family: MHC class I polypeptide-related sequence
• UniProt Gene Name: MICB
• UniProt Synonym Gene Names: MIC-B
• UniProt Entry Name: MICB_HUMAN

NCBI Description

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

MICB: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. The MICB*004 allele is associated with rheumatoid arthritis. Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk. Belongs to the MHC class I family. MIC subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; regulation of immune response; response to retinoic acid; viral reproduction; response to heat; natural killer cell mediated cytotoxicity; cytolysis; immune response-activating cell surface receptor signaling pathway; negative regulation of defense response to virus by host; response to oxidative stress; gamma-delta T cell activation; T cell mediated cytotoxicity