Testing Data #1
(Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR (MBS224569) followed by Goat anti Mouse IgG (H/L):FITC (MBS235142))
Mouse MHC CLASS II DR Monoclonal Antibody | anti-HD59 antibody
MOUSE ANTI BOVINE MHC CLASS II DR:FITC
Applications
Flow Cytometry, Functional Assay
Synonyms
MHC CLASS II DR; Monoclonal Antibody; MOUSE ANTI BOVINE MHC CLASS II DR:FITC; anti-HD59 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CC108
Specificity
This item specifically recognises Bovine MHC Class II DR. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.
The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.
The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Sequence Length
43
Applicable Applications for anti-HD59 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the Mouse NS1 myeloma cell line.
Buffer Solution
Buffer Solution
Target Species
Bovine
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR (MBS224569) followed by Goat anti Mouse IgG (H/L):FITC (MBS235142))
Testing Data #1
(Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR (MBS224569) followed by Goat anti Mouse IgG (H/L):FITC (MBS235142))
Testing Data #2
(Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR:FITC(MBS224409))
Testing Data #2
(Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR:FITC(MBS224409))
Testing Data #3
(Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR:RPE(MBS224588))
Testing Data #3
(Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR:RPE(MBS224588))
Related Product Information for anti-HD59 antibody
MBS224569 specifically recognises Bovine MHC Class II DR. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
4,586 Da
NCBI Official Full Name
MHC class 1 alpha chain
UniProt Protein Name
MHC class 1 alpha chain
UniProt Gene Name
HD59
UniProt Entry Name
P79464_BOVIN
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Product Notes
The MHC CLASS II DR hd59 (Catalog #AAA224409) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MHC CLASS II DR can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the MHC CLASS II DR hd59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC CLASS II DR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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