Mouse MHC CLASS II DQ DR POLYMORPHIC Monoclonal Antibody

MOUSE ANTI SHEEP MHC CLASS II DQ DR POLYMORPHIC:FITC

Cat. Num. AAA215656

• Applications: Flow Cytometry, Functional Assay
• Synonyms: MHC CLASS II DQ DR POLYMORPHIC; Monoclonal Antibody; MOUSE ANTI SHEEP MHC CLASS II DQ DR POLYMORPHIC:FITC; anti-MHC CLASS II DQ DR POLYMORPHIC antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 49.1
• Specificity: The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain. ; This item recognises a polymorphic epitope on ovine MHC class II DQ and DR molecules, which are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma. ; In recent work, this clone was found to recognise ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1.
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)

• Applicable Applications for anti-MHC CLASS II DQ DR POLYMORPHIC antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: 1/10
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Sheep
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR (POLYMORPHIC))

Related Product Information for anti-MHC CLASS II DQ DR POLYMORPHIC antibody

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.MBS215655 recognises a polymorphic epitope on ovine MHC class II DQ and DR molecules, which are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.In recent work, this clone was found to recognise ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1. Please refer to reference 5 (Ballingall, K. et al. 1995) for further information.