Human RuvB-like 1 ELISA Kit | RUVBL1 elisa kit

Human RuvB-like 1 ELISA Kit

Cat. Num. AAA762808

• Gene Names: Ruvbl1; NMP238; Tip49a; Pontin52
• Reactivity: Human
• Synonyms: RuvB-like 1; Human RuvB-like 1 ELISA Kit; RUVBL1/TIP49a/Pontin 52/Nuclear matrix protein 238 (NMP 238)/INO80 complex subunit H/TIP60-associated protein 54-alpha (TAP54-alpha)/49 kDa TATA box-binding protein-interacting protein (49 kDa TBP-interacting protein)/54 kDa erythrocyte cytosolic protein (ECP-54)/; RUVBL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RTN4. No significant cross-reactivity or interference between RTN4 and analogues was observed.
• Sequence Length: 456

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: <46.875pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RTN4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RTN4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RUVBL1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RTN4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RTN4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RTN4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RTN4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 22208848
• NCBI GeneID: 65137
• NCBI Accession #: NP_671706.1
• NCBI GenBank Nucleotide #: NM_147177.1
• Molecular Weight: 50,214 Da
• NCBI Official Full Name: ruvB-like 1
• NCBI Official Synonym Full Names: RuvB-like AAA ATPase 1
• NCBI Official Symbol: Ruvbl1
• NCBI Official Synonym Symbols: NMP238; Tip49a; Pontin52
• NCBI Protein Information: ruvB-like 1
• UniProt Protein Name: RuvB-like 1
• Protein Family: RuvB
• UniProt Gene Name: Ruvbl1
• UniProt Synonym Gene Names: Tip49; Tip49a; 49 kDa TBP-interacting protein
• UniProt Entry Name: RUVB1_RAT

NCBI Description

nuclear DNA helicase that binds TBP; involved in recombination and transcription [RGD, Feb 2006]

Uniprot Description

RUVBL1: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1- CTNNB1 complex. Belongs to the ruvB family. Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Interacts with IGHMBP2. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Helicase; Motility/polarity/chemotaxis

Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; NuA4 histone acetyltransferase complex; nuclear matrix; nucleoplasm; nucleus; protein complex; ribonucleoprotein complex

Molecular Function: ADP binding; ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent 5'-3' DNA helicase activity; ATPase activity; ATPase binding; DNA helicase activity

Biological Process: cell division; DNA duplex unwinding; DNA recombination; DNA repair; mitosis; regulation of growth; regulation of transcription, DNA-dependent; transcription, DNA-dependent